Določanje vsebnosti rastlinskim hormonom podobnih substanc v japonskem dresniku (Fallopia japonica) in kanadski zlati rozgi (Solidago canadensis) s pomočjo biotestov (Cucumis test)

magistrsko delo

Maja Luković (Author), Jana Ambrožič-Dolinšek (Mentor), Terezija Ciringer (Co-mentor)

Abstract

Invazivne tujerodne vrste se hitro razmnožujejo in razširjajo ter izpodrivajo avtohtone vrste. V naši raziskavi smo želeli raziskati vsebnost rastlinskim hormonom podobnih substanc v dveh invazivnih tujerodnih rastlinskih vrstah, kanadski zlati rozgi (Solidago canadensis) in japonskem dresniku (Fallopia japonica). S kumaričnim (Cucumis) biotestom smo ugotavljali vsebnost avksinom in citokininom podobnih substanc v rastlinskem materialu, v različnih razvojnih stadijih rasti, sušenem v sušilniku ali liofilizatorju. V obeh proučevanih invazivnih vrstah smo v materialu, sušenem v liofilizatorju določili nekoliko več hormonom podobnih substanc v primerjavi s sušenim materialom v sušilniku. Kanadska zlata rozga (Solidago canadensis) je vsebovala med 65 \mug in 116 \mug avksinov in med 0 \mug in 50 \mug citokininov na gram suhe mase (SM) rastlin. Japonski dresnik (Fallopia japonica) je vseboval med 63 \mug in 116 \mug avksinov in med 0 \mug in 53 %g citokininov na gram suhe mase (SM) rastline. Največ avksinov in avksinom podobnih substanc imata obe proučevani vrsti v drugem razvojnem stadiju, v katerem so rastline že razvile dolge, še neolesenele poganjke, ter liste in v tretjem razvojnem stadiju, v katerem so bile rastline skoraj popolnoma razvite, z že nekoliko olesenelimi stebli. Največ citokininov in citokininom podobnih substanc smo zaznali v drugem razvojnem stadiju pri obeh rastlinskih vrstah.

Keywords

magistrska dela;Solidago canadensis;kanadska zlata rozga;Fallopia japonica;japonski dresnik;invazivne tujerodne rastline;avksini;indol-3-butanojska kislina (IBA);citokinini;6-benzilaminopurin (BAP);kumarični (Cucumis) biotest;

Data

Language:	Slovenian
Year of publishing:	2019
Typology:	2.09 - Master's Thesis
Organization:	UM FNM - Faculty of Natural Sciences and Mathematics
Publisher:	[M. Luković]
UDC:	577.175.1:581.96(043.2)
COBISS:	24720136
Views:	1017
Downloads:	69
Average score:	0 (0 votes)
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Other data

Secondary language:	English
Secondary title:	Determination of plant hormone-like substances of the japanese knotweed (Fallopia japonica) and canadian goldenrod (Solidago canadensis) with Cucumis bioassay
Secondary abstract:	The invasive alien species rapidly multiply, fast expand and thus displace the native species. The purpose of our thesis was determining the content of plant-hormone-like substances in two invasive alien plant species, the Canadian goldenrod (Solidago canadensis) and Japanese knotweed (Fallopia japonica). We used a cucumis (Cucumis) bioassay to establish the content of auxins-like and cytokinins-like substances in material in four different developmental stages, dryed in a dryer or freeze dryer. In both invasive species studied, there were more hormone-like substances in the material dried in the liofilizator than in a freeze dryer. The Canadian goldenrod (Solidago canadensis) contained between 65 \mug and 116 \mug of auxins-like and between 0 \mug and 50 \mug of cytokinins-like substances per gram of dry weight (DW). The Japanese knotweed (Fallopia japonica) contained between 63 \mug and 116 \mug of auxins-like and between 0 \mug and 53 \mug of cytokinins-like substances per gram of dry weight (DW). The content of auxins-like and cytokinins-like substances in both studied species was higher in the second growth stage in which the plants had already developed long, yet not lignificated shoots and leaves, as well as in the third developmental stage, in which the plants had been almost fully developed with slightly lignified stem. We noticed that the content of auxins-like and cytokinins-like substances was the highest in the second developmental stage of both plant species.
Secondary keywords:	master theses;Solidago canadensis;Canadian goldenrod;Fallopia japonica;Japanese knotweed;invasive alien species;auxins;indol-3-butyric acid (IBA);cytokinins;6-benzylaminopurine (BAP);Cucumis bioassay;
Type (COBISS):	Master's thesis/paper
Thesis comment:	Univ. v Mariboru, Fak. za naravoslovje in matematiko, Oddelek za biologijo
Pages:	XIII, 67 f.
ID:	11103177