diplomsko delo
Brigita Valentinčič (Author), Polona Jamnik (Reviewer), Barbara Jeršek (Mentor)

Abstract

Namen diplomskega dela je bil ugotoviti, ali izbrana eterična olja (EO) delujejo protiglivno. Zato smo jim določili minimalno inhibitorno koncentracijo (MIC) ter minimalno mikrobicidno koncentracijo (MBC) ter njihovo delovanje primerjali z že uveljavljenim razkužilom – vodikovim peroksidom. Testirali smo EO origana (Origanum vulgare), grške bazilike (Ocimum basilicum), sladkega pelina (Artemisia annua), sivke (Lavandula hybrida), laškega smilja (Helichrysum italicum), rožmarina (Rosmarinus officinalis), nemške kamilice (Matricaria recutita) in žajblja (Salvia officinalis) ter vodikov peroksid s kvasovkami vrste Candida albicans in plesnimi vrste Penicillium griseofulvum. Za testiranje protiglivne aktivnosti smo uporabili metodo razredčevanja v mikrotitrski ploščici. Natančne vrednosti MIC in MBC smo za kvasovke določili z metodo štetja kolonij na trdnem gojišču, za plesni pa s pregledovanjem gojišča s plesnimi v mikrotitrskih ploščicah pod lupo. Kvasovke vrste C. albicans so bile bolj dovzetne za EO kot plesni vrste P. griseofulvum. Kvasovkam smo vrednosti MIC določili v območju <0,78–6,25 mg/ml, plesnim pa v območju 3,13–12,5 mg/ml. EO origana je rast obeh gliv inhibiralo pri najnižji koncentraciji med testiranimi EO. Vodikov peroksid je rast kvasovk vrste C. albicans inhibiral pri nižji koncentraciji kot eterična olja, medtem ko je bila vrednost MIC za plesni vrste P. griseofulvum primerljiva z vrednostjo MIC EO origana. Z eksperimentalnim delom smo prišli do zaključka, da imajo eterična olja protiglivno delovanje, in sicer fungicidno aktivnost. Pri tem moramo upoštevati, da so posamezne vrste eteričnih olj različno učinkovite in da so posamezne vrste gliv zanje različno dovzetne. Rezultati kažejo, da bi nadaljnje podrobnejše preiskave eteričnih olj in različnih vrst gliv lahko vodile do uporabe eteričnih olj kot naravnih protimikrobnih snovi v živilstvu.

Keywords

eterična olja;Origanum vulgare;Ocimum basilicum;Artemisia annua;Lavandula hybrida;Helichrysum italicum;Rosmarinus officinalis;Matricaria recutita;Salvia officinalis;vodikov peroksid;protiglivno delovanje;minimalna inhibitorna koncentracija;kvasovke;Candida albicans;plesni;Penicillium griseofulvum;

Data

Language: Slovenian
Year of publishing:
Typology: 2.11 - Undergraduate Thesis
Organization: UL BF - Biotechnical Faculty
Publisher: [B. Valentinčič]
UDC: 579.24:582.28:615.282:547.913
COBISS: 5068408 Link will open in a new window
Views: 780
Downloads: 287
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Other data

Secondary language: English
Secondary title: Antifungal activity of selected essential oils
Secondary abstract: The aim of our research was to determine whether selected essential oils (EO) exhibit antifungal activity and to determine their minimum inhibitory concentration (MIC) and minimum microbicidal concentration (MBC). We also wanted to compare the antifungal activity of EO with that of an already established disinfectant – hydrogen peroxide. In the study we tested EO of oregano (Origanum vulgare), basil (Ocimum basilicum), sweet wormwood (Artemisia annua), lavander (Lavandula hybrida), Italian strawflower (Helichrysum italicum), rosemary (Rosmarinus officinalis), camomile (Matricaria recutita) and sage (Salvia officinalis) along with hydrogen peroxide against Candida albicans and Penicillium griseofulvum. Broth microdilution method was used to determine the antifungal activity of selected substances. To obtain the exact MIC and MBC for C. albicans we carried out the standard plate count method. To obtain the exact MIC and MBC for P. griseofulvum we inspected media with mould in microtiter plates under a magnifying lens. C. albicans was more susceptible to EO, with MIC values ranging from <0.78 to 6.25 mg/ml, in comparison with P. griseofulvum, with MIC values ranging from 3.13 to 12.5 mg/ml. The EO of oregano inhibited the growth of both fungi at the lowest concentration. Hydrogen peroxide inhibited the growth of C. albicans at a lower concentration than essential oils, while the MIC of hydrogen peroxide for P. griseofulvum was comparable to the MIC of EO of oregano. We can conclude that essential oils do exhibit antifungal activity; however, we have to consider the differences in activity of various types of essential oils and the fact that different species of fungi show different degrees of susceptibility to them. The results show that further studies of essential oils and different species of fungi could lead to the use of essential oils as a natural antimicrobial agent in food industry.
Secondary keywords: essential oils;Origanum vulgare;Ocimum basilicum;Artemisia annua;Lavandula hybrida;Helichrysum italicum;Rosmarinus officinalis;Matricaria recutita;Salvia officinalis;hydrogen peroxide;antifungal activity;minimum inhibitory concentration;yeast;Candida albicans;mould;Penicillium griseofulvum;
Type (COBISS): Bachelor thesis/paper
Study programme: 0
Embargo end date (OpenAIRE): 1970-01-01
Thesis comment: Univ. v Ljubljani, Biotehniška fak., Oddelek za živilstvo
Pages: X, 22 f., [4] str. pril.
ID: 11162714