diplomsko delo
Matija Pirc (Author), Aleš Podgornik (Mentor)

Abstract

Princip dvofaznega vodnega sistema se uporablja za frakcionacijo tekoče-tekoče, ki se je zaradi uporabe pri ekstrakciji, ločevanju, čiščenju in obogatitvi beljakovin, membran, virusov in drugih biomolekul razširila tako na laboratorijskem nivoju kot tudi v industriji. Ključne lastnosti tega načina separacije so prijazno okolje za biološke makromolekule, visok izkoristek in možnost enostavnega prenosa procesa na proizvodni nivo (»scale up«), prav tako pa je okolju prijazna ter finančno ugodna metoda . Opisali bomo njene temeljne lastnosti, postopek separacije faz in termodinamske osnove dvofaznih vodnih sistemov.

Keywords

dvofazni vodni sistem;polimerne raztopine;ekstrakcija biomolekul;separacija faz;termodinamika;fazni diagrami;diplomska dela;

Data

Language: Slovenian
Year of publishing:
Typology: 2.11 - Undergraduate Thesis
Organization: UL FKKT - Faculty of Chemistry and Chemical Technology
Publisher: [M. Pirc]
UDC: 66.08:544.3(043.2)
COBISS: 1538326211 Link will open in a new window
Views: 708
Downloads: 237
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Other data

Secondary language: English
Secondary title: Thermodynamic basis of aqueous two-phase systems
Secondary abstract: The principle of the aqueous two-phase system is used for liquid-liquid fractionation, which has expanded due to its use in extraction, separation, purification and enrichment of proteins, membranes, viruses and other biomolecules, both in laboratory and on industrial level. This separation method provides a friendly environment for macromolecules, high efficiency and the possibility of scaling up the process to an industrial level. It is environmentally friendly and a financially favourable method. We will focus in describing the basic properties of aqueous two-phase systems, the process of phase partitioning and thermodynamic basis of this method.
Secondary keywords: thermodynamics;biomolecule extraction;phase partitioning;
Type (COBISS): Bachelor thesis/paper
Study programme: 1000372
Embargo end date (OpenAIRE): 1970-01-01
Thesis comment: Univ. v Ljubljani, Fak. za kemijo in kemijsko tehnologijo
Pages: 17 str.
ID: 11217781