Filipa A. Vicente (Avtor), Igor Plazl (Avtor), Sónia P. M. Ventura (Avtor), Polona Žnidaršič Plazl (Avtor)

Povzetek

Separation and purification of biomacromolecules either in biopharmaceuticals and fine chemicals manufacturing, or in diagnostics and biological characterization, can substantially benefit from application of microfluidic devices. Small volumes of equipment, very efficient mass and heat transfer together with high process control result in process intensification, high throughputs, low energy consumption and reduced waste production as compared to conventional processing. This review highlights microfluidics-based separation and purification of proteins and nucleic acids with the focus on liquid–liquid extractions, particularly with biocompatible aqueous two-phase systems, which represent a cost-effective and green alternative. A variety of microflow set-ups are shown to enable sustainable and efficient isolation of target biomolecules both for preparative, as well as for analytical purposes.

Ključne besede

mikropretočni sistemi;biomakromolekule;dvofazni vodni sistemi;zaključni procesi;microflow systems;biomacromolecules;aqueous two-phase systems;downstream processing;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.02 - Pregledni znanstveni članek
Organizacija: UL FKKT - Fakulteta za kemijo in kemijsko tehnologijo
UDK: 66.02
COBISS: 1538561987 Povezava se bo odprla v novem oknu
ISSN: 1463-9262
Št. ogledov: 293
Št. prenosov: 110
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: mikropretočni sistemi;biomakromolekule;dvofazni vodni sistemi;zaključni procesi;
Vrsta dela (COBISS): Članek v reviji
Strani: str. 4391-4410
Letnik: ǂVol. ǂ22
Zvezek: ǂiss. ǂ14
Čas izdaje: 21 Jul. 2020
DOI: 10.1039/c9gc04362d
ID: 12907181