diplomsko delo
Terezija Jovanovski (Author), Bojana Bogovič Matijašić (Reviewer), Barbara Jeršek (Mentor)

Abstract

Kot odgovor na vedno večjo potrebo po alternativnih protibakterijskih sredstvih, smo preverjali protibakterijsko delovanje eteričnih olj in hidrolatov petih izbranih zdravilnih rastlin: sladki pelin (Artemisia annua), sivka (Lavandula hybrida), laški smilj (Helichrysum italicum), nemška kamilica (Matricaria recutita) in žajbelj (Salvia officinalis) proti po Gramu negativnim bakterijam vrste Escherichia coli in po Gramu pozitivnim bakterijam vrste Staphylococcus epidermidis. Z metodo razredčevanja v mikrotitrski ploščici smo jim določili minimalno inhibitorno koncentracijo (MIC), z metodo štetja kolonij na trdnem gojišču pa smo potrdili minimalno baktericidno koncentracijo (MBC). Eterična olja so bolje delovala na po Gramu pozitivne bakterije, večina z MIC 3,13 mg/ml. MIC za po Gramu negativne bakterije so bile večinoma 6,25 mg/ml. Najbolje sta proti bakterijam učinkovali eterični olji sivke in laškega smilja, sledila sta sladki pelin in nemška kamilica. Vzorec mešanice eteričnega olja in hidrolata žajblja je imel najbolj spremenljive rezultate. Eterična olja so imela boljši protibakterijski učinek kot hidrolati. V 50 % koncentraciji ni noben inhibiral rasti bakterij. Pri 75 % in 100 % koncentraciji smo ugotovili očitno protibakterijsko delovanje samo pri hidrolatu sivke. Hidrolati sladkega pelina, laškega smilja in nemške kamilice, so sicer pokazali šibko protibakterijsko delovanje v 100 % koncentraciji, a le proti bakterijam vrste S. epidermidis. Rezultati naših eksperimentov kažejo na to, da bi lahko eterična olja in hidrolate zdravilnih rastlin uporabili kot protibakterijska sredstva tudi v živilstvu, za kar pa bi bile potrebne še dodatne raziskave.

Keywords

eterična olja;hidrolati;zdravilne rastline;protibakterijsko delovanje;Escherichia coli;Staphylococcus epidermidis;

Data

Language: Slovenian
Year of publishing:
Typology: 2.11 - Undergraduate Thesis
Organization: UL BF - Biotechnical Faculty
Publisher: [T. Jovanovski]
UDC: 579.24:547.913:633.8
COBISS: 5090424 Link will open in a new window
Views: 740
Downloads: 248
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Other data

Secondary language: English
Secondary title: Antibacterial activity of selected medicinal herbs' hydrosols and essential oils
Secondary abstract: In response to the increasing need for alternative antibacterial agents, we analyzed antibacterial activity of essential oils and hydrosols of five selected medicinal plants: mugwort (Artemisia annua), lavandin (Lavandula hybrida), curry plant (Helichrysum italicum), German chamomile (Matricaria recutita) and sage (Salvia officinalis) against Escherichia coli and Staphylococcus epidermidis. Minimal inhibitory concentration (MIC) was determined using broth microdilution method. Minimal bactericidal concentration (MBC) was confirmed using plate count method. Gram-positive bacteria were more susceptible to essential oils, with most of them having MIC 3.13 mg/ml, while MIC for Gram-negative bacteria were mostly 6.25 mg/ml. Essential oils of lavandin and curry plant were the most effective, followed by mugwort and German chamomile. The sample containing sage essential oil and hydrosol had the most variable results. Essential oils had a better antibacterial effect than hydrosols. At 50 % concentration, hydrosols did not show any inhibition of bacterial growth. At 75 % and 100 % however, antibacterial activity was determined, but only for lavandin hydrosol. Mugwort, curry plant and German chamomile also showed weak antibacterial activity at 100 % concentration, but only against S. epidermidis. Results of our experiments indicate that essential oils and hydrosols of medicinal plants could also be used as antibacterial agents in food industry, which would require further research.
Secondary keywords: essential oils;hydrosols;medicinal herbs;antibacterial activity;Escherichia coli;Staphylococcus epidermidis;
Type (COBISS): Bachelor thesis/paper
Study programme: 0
Thesis comment: Univ. v Ljubljani, Biotehniška fak., Oddelek za živilstvo
Pages: VIII, 24 f.
ID: 11217833