Separacija glikanov s kromatografijo z mešanimi režimi

diplomsko delo

Jona Žohar (Author), Matevž Pompe (Mentor)

Abstract

Glikani so ogljikovi hidrati, ki imajo pomembno strukturno in metabolično vlogo v organizmih, zaradi česar se pogosto uporabljajo tudi v farmacevtski industriji. V diplomskem delu, sem na maltodextrinih razvila metodo za separacijo glikanov s kromatografijo z mešanimi režimi. Vzorec maltodextrinov, na katerem sem razvila metodo, sem označila s fluorescenčnim označevalcem in odvečni označevalec odstranila z ekstrakcijo na trdno fazo. Metodo sem razvila na Thermo Fisher-jevi koloni, GlycanPac AXH-1, na kateri poteka več mehanizmov separacije, in sicer ionske in hidrofilne interakcije. Razvito metodo sem nato validirala z D-glukozo in D-maltozo. Pri čemer smo preverjali linearnost, občutljivost, ponovljivost, točnost in robustnost.

Keywords

glikani;ogljikovi hidrati;2-aminobenzojska kislina;2-AA;ekstrakcija na trdo fazo;SPE;kromatografija z mešanimi režimi;validacija metode;diplomska dela;

Data

Language:	Slovenian
Year of publishing:	2020
Typology:	2.11 - Undergraduate Thesis
Organization:	UL FKKT - Faculty of Chemistry and Chemical Technology
Publisher:	[J. Žohar]
UDC:	543.544(043.2)
COBISS:	30501891
Views:	460
Downloads:	127
Average score:	0 (0 votes)
Metadata:

Other data

Secondary language:	English
Secondary title:	Separation of glycans using mixed-mode chromatography
Secondary abstract:	Glycans are carbohydrates that have an important structural and metabolic role in living organisms, which makes them commonly used in the pharmaceutical industry. As a part of my bachelor's thesis, I developed a method for the separation of glycans using mixed mode chromatography. The method was developed on maltodextrines, which were first labelled with fluorescence 2-AA reagent. I removed the excess of the fluorescence reagent using a solid phase extraction. The method was developed on the Thermo Fisher’s GlycanPac AXH-1 column, which has two mechanisms of separation, namely the ion-exchange and hydrophilic interactions. After its development, the method was validated with D-glucose and D-maltose. In order to validate the method, I checked its linearity, repeatability, sensitivity, accuracy and robustness.
Secondary keywords:	glycans;carbohydrates;mixed-mode chromatography;
Type (COBISS):	Bachelor thesis/paper
Study programme:	1000373
Embargo end date (OpenAIRE):	1970-01-01
Thesis comment:	Univ. v Ljubljani, Fak. za kemijo in kemijsko tehnologijo, UNI Kemija
Pages:	V f., 36 str.
ID:	12031346