diplomsko delo
Maja Čič (Author), Polona Jamnik (Mentor), Polona Jamnik (Thesis defence commission member), Nada Žnidaršič (Thesis defence commission member), Mojca Narat (Thesis defence commission member)

Abstract

Spremljanje celičnega zdravja je pomemben del vzdrževanja celičnih kultur. Določanje števila živih celic poteka s pomočjo testov celične viabilnosti. Spremljanje ostalih parametrov, ki so ključni za normalno funkcionalnost celic pa poteka s pomočjo testov celične vitalnosti. Nekateri od teh parametrov so permeabilnost celične membrane, celični membranski potencial, encimska in metabolna aktivnost, sinteza DNA in celična proliferacija ter stopnja oksidativnega stresa. In vitro teste se lahko klasificira na podlagi načina detekcije merjenega parametra. Te skupine vključujejo teste izključitve barvil, kolorimetrične teste, fluorometrični in luminometrične teste. Testi temeljijo na osnovnih in ključnih biokemijskih reakcijah, ki potekajo v celici, za njihovo izpeljavo pa se uporabljajo reagenti, katerih pretvorbo je preprosto detektirati.

Keywords

celično zdravje;celična viabilnost;celična vitalnost;

Data

Language: Slovenian
Year of publishing:
Typology: 2.11 - Undergraduate Thesis
Organization: UL BF - Biotechnical Faculty
Publisher: [M. Čič]
UDC: 601.2:576.3:576.34/.35(043.2)
COBISS: 31421955 Link will open in a new window
Views: 410
Downloads: 72
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Other data

Secondary language: English
Secondary title: Cell health
Secondary abstract: Monitoring cell health is an important part of sustaining cell culture growth. To determine live cell number, cell viability assays are used. Measuring other parameters, crucial for normal cell functioning, is done with cell vitality assays. Some of these parameters are cell membrane permeability, cell membrane potential, enzymatic and metabolic activity, DNA synthesis and cellular proliferation and also the rate of oxidative stress. In vitro assays are classified according to the measuring method. These groups are dye exclusion assays, colorimetric, fluorimetric and luminometric assays. Cell assays are based on vital intracellular biochemical reactions and reagents with conversions simple to detect.
Secondary keywords: cell health;cell viability;cell vitality;
Type (COBISS): Bachelor thesis/paper
Study programme: 0
Embargo end date (OpenAIRE): 1970-01-01
Thesis comment: Univ. v Ljubljani, Biotehniška fak., Študij biotehnologije
Pages: VI, 22 str.
ID: 12038905