Beatriz Sanz-Sagué (Avtor), Amaia Sáenz-Hernández (Avtor), Bojana Žegura (Avtor), Alja Štraser (Avtor), Katja Kološa (Avtor), Iza Rozman (Avtor)

Povzetek

The applications of magnetic nanoparticles (MNPs) as biocatalysts in different biomedical areas have been evolved very recently. One of the main challenges in this field is to design affective MNPs surfaces with catalytically active atomic centres, while producing minimal toxicological side effects on the hosting cell or tissues. MNPs of vanadium spinel ferrite (VFe2O4) are a promising material for mimicking the action of natural enzymes in degrading harmful substrates due to the presence of active V5+ centres. However, the toxicity of this material has not been yet studied in detail enough to grant biomedical safety. In this work, we have extensively measured the structural, compositional, and magnetic properties of a series of VxFe3-xO4 spinel ferrite MNPs to assess the surface composition and oxidation state of V atoms, and also performed systematic and extensive in vitro cytotoxicity and genotoxicity testing required to assess their safety in potential clinical applications. We could establish the presence of V5+ at the particle surface even in water-based colloidal samples at pH 7, as well as different amounts of V2+ and V3+ substitution at the A and B sites of the spinel structure. All samples showed large heating efficiency with Specific Loss Power values up to 400 W/g (H0 = 30 kA/m; f = 700 kHz). Samples analysed for safety in human hepatocellular carcinoma (HepG2) cell line with up to 24h of exposure showed that these MNPs did not induce major genomic abnormalities such as micronuclei, nuclear buds, or nucleoplasmic bridges (MNIs, NBUDs, and NPBs), nor did they cause DNA double-strand breaks (DSBs) or aneugenic effects—types of damage considered most harmful to cellular genetic material. The present study is an essential step towards the use of these type of nanomaterials in any biomedical or clinical application.

Ključne besede

magnetni nanodelci;vanadijev ferit;citotoksičnost;genotoksičnost;specifična absorpcijska moč;celična živost;magnetic nanoparticles;vanadium ferrite;cytotoxicity;genotoxicity;specific power absorption;cell viability;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: NIB - Nacionalni inštitut za biologijo
UDK: 577
COBISS: 190594819 Povezava se bo odprla v novem oknu
ISSN: 0009-2797
Št. ogledov: 24
Št. prenosov: 9
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: magnetni nanodelci;vanadijev ferit;citotoksičnost;genotoksičnost;specifična absorpcijska moč;celična živost;
Komentar vira: Soavtorji: Amaia Sáenz- Hernández, A. C. Moreno Maldonado, Jesús A. Fuentes-García, Jorge M. Nuñez, Bojana Žegura, Alja Štern, Katja Kološa, Iza Rozman, Teobaldo E. Torres, Gerardo F. Goya;
Strani: str. [1]-13
Letnik: ǂVol. ǂ394
Zvezek: ǂ[art. no.] ǂ110977
Čas izdaje: 1 May 2024
DOI: 10.1016/j.cbi.2024.110977
ID: 23769236