Secondary abstract: |
Astrocytes, the most abundant cells within the human brain were far long considered as
passive, connective tissue. Nowadays, we are aware that astrocytes have a complex function
in different phases of brain development and maturation. They also actively participate in the
inactivation of neurotransmitters. Thus they express different transporters and enzymes,
which take part in the metabolism of neurotransmitters. Histamine transport has not been
elucidated, yet. Nevertheless, it has been reported that astrocytes take up histamine.
Neurotransmitter histamine is involved in many brain functions and ethiopatogenesis of many
neurological diseases. Histaminergic neurons originate in the tuberomammillary nucleus of
posterior basal hypothalamus and project into different brain and spinal regions. The density
of histaminergic innervations is brain-region dependent. Cortex and striatum possess more
histaminergic innervations than cerebellum. Due to this fact and brain-region dependent role
of histamine, we decided to investigate the characteristics of histamine uptake in astrocytes
raised from rat striatum and cerebellum. We focused our study toward kinetic and
pharmacological properties of histamine transport in primary astrocytes cultures, raised from
newborn rat cerebellum and striatum. Next goal was to check whether the possible role of
OCT (OCT-organic cation transporter) in histamine innervations. We investigated time and
concentrations-dependent kinetic parameters and the influence of different compounds on
histamine uptake.
Our results showed that histamine is taken up into astrocytes from both brain regions by two
different mechanisms. There is a big difference of histamine uptake into astrocytes from
different brain-regions. Histamine uptake is higher into striatal astrocytes. Organic cation
transporters are not involved in histamine transport. The rate and affinity of histamine uptake
is significantly higher for striatal than in cerebellar astrocytes. Desipramine, inhibits the
uptake of histamine only into striatal astrocytes, but has no influence on histamine uptake into
glial cells from cerebellum.
Our results provide additional evidence for the brain-region specific function of astrocytes. |