Vpliv možganskega področja na lastnosti privzema histamina v astrocite

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Jezik:	Slovenski jezik
Leto izida:	2010
Izvor:	Ljubljana
Tipologija:	2.11 - Diplomsko delo
Organizacija:	UL FFA - Fakulteta za farmacijo
Založnik:	[A. Raztresen]
UDK:	577
COBISS:	2893169
Št. ogledov:	942
Št. prenosov:	265
Ocena:	0 (0 glasov)
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Sekundarni jezik:	Angleški jezik
Sekundarni naslov:	Brain region-dependent characteristics of histamine transport into astrocytes
Sekundarni povzetek:	Astrocytes, the most abundant cells within the human brain were far long considered as passive, connective tissue. Nowadays, we are aware that astrocytes have a complex function in different phases of brain development and maturation. They also actively participate in the inactivation of neurotransmitters. Thus they express different transporters and enzymes, which take part in the metabolism of neurotransmitters. Histamine transport has not been elucidated, yet. Nevertheless, it has been reported that astrocytes take up histamine. Neurotransmitter histamine is involved in many brain functions and ethiopatogenesis of many neurological diseases. Histaminergic neurons originate in the tuberomammillary nucleus of posterior basal hypothalamus and project into different brain and spinal regions. The density of histaminergic innervations is brain-region dependent. Cortex and striatum possess more histaminergic innervations than cerebellum. Due to this fact and brain-region dependent role of histamine, we decided to investigate the characteristics of histamine uptake in astrocytes raised from rat striatum and cerebellum. We focused our study toward kinetic and pharmacological properties of histamine transport in primary astrocytes cultures, raised from newborn rat cerebellum and striatum. Next goal was to check whether the possible role of OCT (OCT-organic cation transporter) in histamine innervations. We investigated time and concentrations-dependent kinetic parameters and the influence of different compounds on histamine uptake. Our results showed that histamine is taken up into astrocytes from both brain regions by two different mechanisms. There is a big difference of histamine uptake into astrocytes from different brain-regions. Histamine uptake is higher into striatal astrocytes. Organic cation transporters are not involved in histamine transport. The rate and affinity of histamine uptake is significantly higher for striatal than in cerebellar astrocytes. Desipramine, inhibits the uptake of histamine only into striatal astrocytes, but has no influence on histamine uptake into glial cells from cerebellum. Our results provide additional evidence for the brain-region specific function of astrocytes.
Vrsta dela (COBISS):	Diplomsko delo
Strani:	XI, 58 f.
ID:	12061570