Amelie Detterbeck (Author), Paula Pongrac (Author), Daniel Persson (Author), Katarina Vogel-Mikuš (Author), Mitja Kelemen (Author), Primož Vavpetič (Author), Primož Pelicon (Author), Iztok Arčon (Author), Søren Husted (Author), Jan Kofod Schjoerring (Author), Stephan Clemens (Author)

Abstract

Breeding and engineering of biofortified crops will benefit from a better understanding of bottlenecks controlling micronutrient loading within the seeds. However, few studies have addressed the changes in micronutrient concentrations, localization, and speciation occurring over time. Therefore, we studied spatial patterns of zinc and iron accumulation during grain development in two barley lines with contrasting grain zinc concentrations. Microparticle-induced-X-ray emission and laser ablationinductively coupled plasma mass spectrometry were used to determine tissue-specific accumulation of zinc, iron, phosphorus, and sulfur. Differences in zinc accumulation between the lines were most evident in the endosperm and aleurone. A gradual decrease in zinc concentrations from the aleurone to the underlying endosperm was observed, while iron and phosphorus concentrations decreased sharply. Iron co-localized with phosphorus in the aleurone, whereas zinc co-localized with sulfur in the sub-aleurone. We hypothesize that differences in grain zinc are largely explained by the endosperm storage capacity. Engineering attempts should be targeted accordingly.

Keywords

barley (Hordeum vulgare L.);biofortification;grain development;grain loading;LA-ICP-MS;μ-PIXE;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UNG - University of Nova Gorica
UDC: 53
COBISS: 33245443 Link will open in a new window
ISSN: 0021-8561
Views: 2051
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Other data

URN: URN:SI:UNG
Type (COBISS): Not categorized
Pages: 12 str.
Chronology: [in press] 2020
DOI: 10.1021/acs.jafc.0c04833
ID: 12088805