Amelie Detterbeck (Avtor), Paula Pongrac (Avtor), Daniel Persson (Avtor), Katarina Vogel-Mikuš (Avtor), Mitja Kelemen (Avtor), Primož Vavpetič (Avtor), Primož Pelicon (Avtor), Iztok Arčon (Avtor), Søren Husted (Avtor), Jan Kofod Schjoerring (Avtor), Stephan Clemens (Avtor)

Povzetek

Breeding and engineering of biofortified crops will benefit from a better understanding of bottlenecks controlling micronutrient loading within the seeds. However, few studies have addressed the changes in micronutrient concentrations, localization, and speciation occurring over time. Therefore, we studied spatial patterns of zinc and iron accumulation during grain development in two barley lines with contrasting grain zinc concentrations. Microparticle-induced-X-ray emission and laser ablationinductively coupled plasma mass spectrometry were used to determine tissue-specific accumulation of zinc, iron, phosphorus, and sulfur. Differences in zinc accumulation between the lines were most evident in the endosperm and aleurone. A gradual decrease in zinc concentrations from the aleurone to the underlying endosperm was observed, while iron and phosphorus concentrations decreased sharply. Iron co-localized with phosphorus in the aleurone, whereas zinc co-localized with sulfur in the sub-aleurone. We hypothesize that differences in grain zinc are largely explained by the endosperm storage capacity. Engineering attempts should be targeted accordingly.

Ključne besede

barley (Hordeum vulgare L.);biofortification;grain development;grain loading;LA-ICP-MS;μ-PIXE;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UNG - Univerza v Novi Gorici
UDK: 53
COBISS: 33245443 Povezava se bo odprla v novem oknu
ISSN: 0021-8561
Št. ogledov: 2051
Št. prenosov: 0
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

URN: URN:SI:UNG
Vrsta dela (COBISS): Delo ni kategorizirano
Strani: 12 str.
Čas izdaje: [in press] 2020
DOI: 10.1021/acs.jafc.0c04833
ID: 12088805