Nitrosative stress in the frontal cortex from dogs with canine cognitive dysfunction

Sonja Prpar (Author), Maja Zakošek (Author), Malan Štrbenc (Author), Boris Rogelj (Author), Gregor Majdič (Author)

Abstract

Canine cognitive dysfunction (CCD) is an age-related disorder similar to human Alzheimer's disease (AD) that occurs in elderly dogs. Nitrosative stress has been implicated as one of the causes leading to neurodegenerative diseases, particularly AD. Its involvement in the development of CCD has not been studied so far. In the present study, immunohistochemical staining detected all three isoforms of nitric oxide synthases (nNOS, eNOS, and iNOS) and 3-nitrotyrosine (3-NT) in brains from CCD-affected dogs and non-demented control dogs in all layers of the canine frontal cortex. In CCD-affected and non-demented brains, nNOS was highly expressed in pyramidal-like neurons in the upper cortical layers. nNOS has also been observed in astrocytes in the CCD frontal cortex. The nNOS immunohistochemical staining was statistically significantly elevated in dogs with CCD in comparison to non-demented dogs. Blood vessel wall cells were positive for eNOS, which was also expressed in astrocytes and neurons. Intense 3-NT immunoreactivity was observed in the upper cortical layers, where amyloid-beta deposits spread in the last stage of CCD. Brain cells in the same area were highly immunoreactive for iNOS. This infers that neuroinflammation and nitrosative stress might exacerbate the neurodegenerative process in CCD-affected brains, ultimately leading to cognitive impairment.

Keywords

canine cognitive dysfunction;nitrosative stress;nNOS;eNOS;iNOS;3-nitrotyrosine;cognitive dysfunction;brain;dogs;

Data

Language:	English
Year of publishing:	2020
Typology:	1.01 - Original Scientific Article
Organization:	UL VF - Veterinary Faculty
UDC:	636.7.09:616.89
COBISS:	38304259
ISSN:	2297-1769
Views:	1210
Downloads:	153
Average score:	0 (0 votes)
Metadata:

Other data

Secondary language:	Slovenian
Secondary keywords:	Nitrosative Stress;Cognitive Dysfunction;Brain;Dogs;
Type (COBISS):	Article
Pages:	art. 573155, str. 1-14
Issue:	ǂVol. ǂ7
Chronology:	2020
DOI:	10.3389/fvets.2020.573155
ID:	12174530