magistrsko delo
Lea Babič (Author), Damjana Drobne (Reviewer), Mojca Narat (Mentor), Iva Hafner Bratkovič (Co-mentor)

Abstract

V sklopu te magistrske naloge smo raziskali vplive treh tipov nanodelcev na celično linijo mišjih možganskih makrofagov (mikroglijske celice) in na nevronsko celično linijo. Z dodajanjem CM-H2DCFDA barvila v medij smo spremljali sproščanje reaktivnih kisikovih spojin. Sproščanje citokinov smo pa smo detektirali z encimsko imunoadsorpcijsko metodo (ELISA). Po prekonočni inkubaciji celic z nanodelci smo s pregledom s presevnim elektronskim mikroskopom opazili internalizacijo nanodelcev za vse tipe nanodelcev pri izbranih koncentracijah. Ugotovili smo, da ob kratkotrajni izpostavljenosti celic testiranim nanodelcem pri izbranih koncentracijah le ti niso imeli direktnega vpliva na viabilnost celic pri nobeni celični liniji, kar smo preverjali z barvanjem celic s propidijevim jodidom, ki ob kratkotrajni inkubaciji vstopa le v mrtve celice. Prišli smo do zaključka, da ti trije tipi nanodelcev v danih koncentracijah ne vplivajo na viabilnost celic, hkrati pa tudi celice ne sproščajo povišanih količin citokinov in reaktivnih kisikovih spojin, ki bi lahko posredno poškodovale katere celice, opazna pa je internalizacija nanodelcev.

Keywords

mikroglia celice;mišje mikroglia celice;nanodelci;preživetje;detekcija signalnih molekul;imunološke metode;ROS;ELISA;

Data

Language: Slovenian
Year of publishing:
Typology: 2.09 - Master's Thesis
Organization: UL BF - Biotechnical Faculty
Publisher: [L. Babič]
UDC: 577.2:620.3:57.083
COBISS: 50002435 Link will open in a new window
Views: 434
Downloads: 72
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Other data

Secondary language: English
Secondary title: In vitro toxicity of nanoparticles in murine microglial cells
Secondary abstract: Despite the fact that the field of research of nanoparticle cytotoxicity is relatively wide, not much is known about the mechanisms of interactions between cells and nanoparticles. In the scope of this master's thesis we investigated interactions between nanoparticles and two cell lines, microglial cells and a neuronal cell line. We observed that the nanoparticles do not make an impact on the viability of both cell lines in short term incubation. We also followed the release of cytokines with enzyme-linked immunosorbent assay (ELISA) when microglial cells were incubated with nanoparticles and measured the reactive oxygen species formation, but we found that there were no significant increases in detection of both types of signalling molecules. Nevertheless, were able to prove that both cell lines are susceptible to internalization of nanoparticles as observed under the transmission electron microscope, which suggests that prolonged incubation with nanoparticles might lead to adverse effects.
Secondary keywords: microglia cells;mice microglia cells;nanoparticles;viability;detection of signalling moleculles;immunological methods;ROS;ELISA;
Type (COBISS): Master's thesis/paper
Study programme: 0
Embargo end date (OpenAIRE): 1970-01-01
Thesis comment: Univ. v Ljubljani, Biotehniška fak., Študij mikrobiologije
Pages: X, 54 f.
ID: 12518897