Marko Tramšek (Author), Andreja Goršek (Author)

Abstract

This article describes experimental determination of the relative impact of significant process parameters that influence volumetric oxygen mass transfer coefficient (kLa) using Taguchi design methodology. For this purpose an automated RC1 reaction calorimeter (Mettler-Toledo), which was originally developed for chemical processes, was modified for the bioprocesses. Simple fermentation using Baker's yeast was studied to illustrate the design procedure. Orthogonal array L25 was selected for the proposed design and ANOVA method was used for recognizing the relative influence of the process parameters. Within the observed range of temperature (?), fermentation media volume (VFM), and yeast mass concentration (?Y), these process parameters were found to be unimportant compared to the volumetric air flow rate (qV,a) and rotational frequency of the stirrer (fm). The qV,a had a substantial effect on the kLa value (89.2 %) and the fm had just a small one (3.6 %), meanwhile the remain fraction to 100 % represents error. The results refer strictly to the selected case study. Anyhow, the proposed procedure shows that application of the Taguchi approach for analyzing the oxygen mass transfer based on the experimental data obtained from a highly-automated laboratory reactor appears to have potential usage in general biopharmaceutical process design.

Keywords

bioprocesni parametri;volumetrični prenos kisika;procesni parametri;metoda Taguchi;bioprocesi;analiza variance;bioprocess parameters;mass transfer;volumetric oxygen mass transfer coefficient;process parameters;Taguchi method;analysis of variance;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UM FKKT - Faculty of Chemistry and Chemical Engineering
UDC: 577:66.023
COBISS: 11991574 Link will open in a new window
ISSN: 1226-086X
Views: 1969
Downloads: 39
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Other data

Secondary language: English
Secondary title: Determination of the process parameters relative influence on kLa value using Taguchi design methodology
Secondary keywords: bioprocesni parametri;volumetrični prenos kisika;procesni parametri;metoda Taguchi;bioprocesi;analiza variance;
URN: URN:SI:UM:
Pages: str. 1054-1061
Volume: ǂVol. ǂ13
Issue: ǂno. ǂ7
Chronology: 2007
Keywords (UDC): mathematics;natural sciences;naravoslovne vede;matematika;biological sciences in general;biologija;material bases of life;biochemistry;molecular biology;biophysics;biokemija;molekularna biologija;biofizika;applied sciences;medicine;technology;uporabne znanosti;medicina;tehnika;chemical technology;chemical and related industries;kemijska tehnologija;kemijske in sorodne industrije;chemical processing operations and equipment in general;kemijska procesna tehnika in oprema na splošno;
ID: 13204