Determination of the process parameters relative influence on k[sub]La value using Taguchi design methodology

Marko Tramšek (Avtor), Andreja Goršek (Avtor)

Povzetek

This article describes experimental determination of the relative impact of significant process parameters that influence volumetric oxygen mass transfer coefficient (kLa) using Taguchi design methodology. For this purpose an automated RC1 reaction calorimeter (Mettler-Toledo), which was originally developed for chemical processes, was modified for the bioprocesses. Simple fermentation using Baker's yeast was studied to illustrate the design procedure. Orthogonal array L25 was selected for the proposed design and ANOVA method was used for recognizing the relative influence of the process parameters. Within the observed range of temperature (?), fermentation media volume (VFM), and yeast mass concentration (?Y), these process parameters were found to be unimportant compared to the volumetric air flow rate (qV,a) and rotational frequency of the stirrer (fm). The qV,a had a substantial effect on the kLa value (89.2 %) and the fm had just a small one (3.6 %), meanwhile the remain fraction to 100 % represents error. The results refer strictly to the selected case study. Anyhow, the proposed procedure shows that application of the Taguchi approach for analyzing the oxygen mass transfer based on the experimental data obtained from a highly-automated laboratory reactor appears to have potential usage in general biopharmaceutical process design.

Ključne besede

bioprocesni parametri;volumetrični prenos kisika;procesni parametri;metoda Taguchi;bioprocesi;analiza variance;bioprocess parameters;mass transfer;volumetric oxygen mass transfer coefficient;process parameters;Taguchi method;analysis of variance;

Podatki

Jezik:	Angleški jezik
Leto izida:	2007
Tipologija:	1.01 - Izvirni znanstveni članek
Organizacija:	UM FKKT - Fakulteta za kemijo in kemijsko tehnologijo
UDK:	577:66.023
COBISS:	11991574
ISSN:	1226-086X
Št. ogledov:	1969
Št. prenosov:	39
Ocena:	0 (0 glasov)
Metapodatki:

Ostali podatki

Sekundarni jezik:	Angleški jezik
Sekundarni naslov:	Determination of the process parameters relative influence on kLa value using Taguchi design methodology
Sekundarne ključne besede:	bioprocesni parametri;volumetrični prenos kisika;procesni parametri;metoda Taguchi;bioprocesi;analiza variance;
URN:	URN:SI:UM:
Strani:	str. 1054-1061
Letnik:	ǂVol. ǂ13
Zvezek:	ǂno. ǂ7
Čas izdaje:	2007
Ključne besede (UDK):	mathematics;natural sciences;naravoslovne vede;matematika;biological sciences in general;biologija;material bases of life;biochemistry;molecular biology;biophysics;biokemija;molekularna biologija;biofizika;applied sciences;medicine;technology;uporabne znanosti;medicina;tehnika;chemical technology;chemical and related industries;kemijska tehnologija;kemijske in sorodne industrije;chemical processing operations and equipment in general;kemijska procesna tehnika in oprema na splošno;
ID:	13204