Razvoj kolorimetričnega senzorskega receptorja za zaznavanje biogenih aminov

diplomsko delo univerzitetnega študijskega programa I. stopnje

Gal Bjelovučić (Author), Matjaž Finšgar (Mentor), Tinkara Mastnak (Co-mentor)

Abstract

Diplomsko delo predstavlja razvoj kolorimetričnega senzorskega receptorja (KSR) za zaznavanje trimetilamina, ki skupaj z dimetilaminom in amonijakom tvori enega glavnih parametrov kakovosti hrane ‒ celokupni hlapni bazični dušik ali TVB-N (angl. total volatile basic nitrogen). KSR, ki smo ga razvili, je sestavljen iz izključno z živili združljivih materialov: indikatorja (izvleček vijoličnega korenja) in polimerne osnove (derivat celuloze), ki smo ju v obliki polimerne raztopine nanesli na nosilec iz PET-folije . Za spremljanje barvnih sprememb tako pripravljenega KSR smo pripravili zaprt sistem, ki posnema pogoje v živilski embalaži ter vanj s tehniko vzorčenja iz nezapolnjenega prostora nad vzorcem injicirali analit. Kolorimetrično detekcijo in RGB-analizo smo izvedli s pametnim telefonom in brezplačno dostopno aplikacijo Color Grab. Potrdili smo, da so optične lastnosti indikatorja odvisne od koncentracije trimetilamina in v izbranem koncentracijskem območju dobili barvno lestvico od rdeče-vijolične do temno sive barve. Ugotovitve diplomske naloge so dobro izhodišče za nadaljnje raziskave in izpopolnjevanje tako KSR kot tudi metode. Ker kolorimetrična detekcija s pametnim telefonom ne zahteva usposobljenega osebja in je veliko cenejša ter hitrejša od uveljavljenih instrumentalnih metod, ki se sicer uporabljajo za določanje biogenih aminov, ima ta metoda velik potencial na področju spremljanja kakovosti in varnosti hrane. Morebitna uporaba kolorimetričnih senzorskih receptorjev v sklopu pametnega pakiranja živil bi lahko znatno zmanjšala količino zavržene hrane.

Keywords

kolorimetrični senzorski receptor;biogeni amini;trimetilamin;kontrola kakovosti hrane;diplomske naloge;

Data

Language:	Slovenian
Year of publishing:	2021
Typology:	2.11 - Undergraduate Thesis
Organization:	UM FKKT - Faculty of Chemistry and Chemical Engineering
Publisher:	[G. Bjelovučić]
UDC:	577.354:547.233(043.2)
COBISS:	80075011
Views:	313
Downloads:	37
Average score:	0 (0 votes)
Metadata:

Other data

Secondary language:	English
Secondary title:	Development of a colorimetric sensor receptor for the detection of biogenic amines
Secondary abstract:	The bachelor thesis presents the development of a colorimetric sensor receptor (CSR) for the detection of trimethylamine. Along with dimethylamine and ammonia, this analyte forms one of the main food quality parameters – total volatile basic nitrogen (TVB-N). The developed CSR consists of entirely food-compatible materials: an indicator (black carrot extract) and a polymer matrix (cellulose derivative), from which a polymer solution was prepared to later coat a solid-state substrate (a PET-foil). For the purpose of monitoring the color changes of CSR, a closed system that mimics conditions in food packaging was created and analyte injected according to the headspace method. Colorimetric detection and RGB analysis were performed using a smartphone and a commercially available application Color Grab. We confirmed that optical properties of the indicator depend on the concentration of trimethylamine and observed visible color changes from red-purple to dark gray. The presented findings are a good starting point for further improvement of the CSR and the method. Smartphone-based colorimetric detection does not require trained personnel and is much cheaper and faster than conventional instrumental techniques for the determination of biogenic amines. Therefore, sensing systems compatible with smartphone-based colorimetric detection offer a promising solution for monitoring of food quality and food safety. CSR integrated into smart food packaging have the potential to significantly reduce the amount of food waste.
Secondary keywords:	colorimetric sensor receptor;biogenic amines;trimethylamine;food quality control;
Type (COBISS):	Bachelor thesis/paper
Thesis comment:	Univ. v Mariboru, Fak. za kemijo in kemijsko tehnologijo
Pages:	VIII, 33 str.
ID:	13273622