Tilen Kopač (Author), Ana Lisac (Author), Rok Mravljak (Author), Aleš Ručigaj (Author), Matjaž Krajnc (Author), Aleš Podgornik (Author)

Abstract

The role of bacteriophage therapy in medicine has recently regained an important place. Oral phage delivery for gastrointestinal treatment, transport through the stomach, and fast release in the duodenum is one of such applications. In this work, an efficient polyHIPE/hydrogel system for targeted delivery of bacteriophages with rapid release at the target site is presented. T7 bacteriophages were encapsulated in low crosslinked anionic nanocellulose-based hydrogels, which successfully protected phages at pH < 3.9 (stomach) and completely lost the hydrogel network at a pH above 3.9 (duodenum), allowing their release. Hydrogels with entrapped phages were crosslinked within highly porous spherical polyHIPE particles with an average diameter of 24 µm. PolyHIPE scaffold protects the hydrogels from mechanical stimuli during transport, preventing the collapse of the hydrogel structure and the unwanted phage release. On the other hand, small particle size, due to the large surface-to-volume ratio, enables rapid release at the target site. As a consequence, a fast zero-order release was achieved, providing improved patient compliance and reduced frequency of drug administration. The proposed system therefore exhibits significant potential for a targeted drug delivery in medicine and pharmacy.

Keywords

TEMPO nanoceluloza;bakteriofag T7;inkapsulacija;difuzija učinkovin;sproščanje ničtega reda;TEMPO nanocellulose;T7 bacteriophage;encapsulation;drug diffusion;zero-order release;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UL FKKT - Faculty of Chemistry and Chemical Technology
UDC: 678:615:578.81
COBISS: 73830403 Link will open in a new window
ISSN: 2073-4360
Views: 318
Downloads: 88
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Other data

Secondary language: Slovenian
Secondary keywords: TEMPO nanoceluloza;bakteriofag T7;inkapsulacija;difuzija učinkovin;sproščanje ničtega reda;
Type (COBISS): Article
Pages: str. 1-12
Volume: ǂVol. ǂ13
Issue: ǂiss. ǂ16
Chronology: Aug. 2021
DOI: 10.3390/polym13162648
ID: 13275173