Emilia Szymańska (Author), M. Szekalska (Author), Robert Czarnomysy (Author), Zoran Lavrič (Author), Stanko Srčič (Author), Wojciech Miltyk (Author), K. Winnicka (Author)

Abstract

Chitosan microparticulate delivery systems containing clotrimazole were prepared by a spray drying technique using glycerol 2-phosphate as an ion cross-linker. The impact of a cross-linking ratio on microparticle characteristics was evaluated. Drug-free and drug-loaded unmodified or ion cross-linked chitosan microparticles were examined for the in vitro cytotoxicity in VK2/E6E7 human vaginal epithelial cells. The presence of glycerol 2-phosphate influenced drug loading and encapsulation efficacy in chitosan microparticles. By increasing the cross-linking ratio, the microparticles with lower diameter, moisture content and smoother surface were observed. Mucoadhesive studies displayed that all formulations possessed mucoadhesive properties. The in vitro release profile of clotrimazole was found to alter considerably by changing the glycerol 2-phosphate/chitosan ratio. Results from cytotoxicity studies showed occurrence of apoptotic cells in the presence of chitosan and ion cross-linked chitosan microparticles, followed by a loss of membrane potential suggesting that cell death might go through the mitochondrial apoptotic pathway.

Keywords

hitosan;mikrodelci;citotoksičnost;dostavni sistem;glicerol.2-fosfat;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UL FFA - Faculty of Pharmacy
UDC: 577.1
COBISS: 4190065 Link will open in a new window
ISSN: 1660-3397
Views: 167
Downloads: 64
Average score: 0 (0 votes)
Metadata: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Other data

Secondary language: Slovenian
Secondary keywords: hitosan;mikrodelci;citotoksičnost;dostavni sistem;glicerol 2-fosfat;
Type (COBISS): Article
Pages: 22 str.
Volume: ǂVol. ǂ14
Issue: ǂno. ǂ10
Chronology: 2016
DOI: 10.3390/md14100174
ID: 13576489