Emilia Szymańska (Avtor), M. Szekalska (Avtor), Robert Czarnomysy (Avtor), Zoran Lavrič (Avtor), Stanko Srčič (Avtor), Wojciech Miltyk (Avtor), K. Winnicka (Avtor)

Povzetek

Chitosan microparticulate delivery systems containing clotrimazole were prepared by a spray drying technique using glycerol 2-phosphate as an ion cross-linker. The impact of a cross-linking ratio on microparticle characteristics was evaluated. Drug-free and drug-loaded unmodified or ion cross-linked chitosan microparticles were examined for the in vitro cytotoxicity in VK2/E6E7 human vaginal epithelial cells. The presence of glycerol 2-phosphate influenced drug loading and encapsulation efficacy in chitosan microparticles. By increasing the cross-linking ratio, the microparticles with lower diameter, moisture content and smoother surface were observed. Mucoadhesive studies displayed that all formulations possessed mucoadhesive properties. The in vitro release profile of clotrimazole was found to alter considerably by changing the glycerol 2-phosphate/chitosan ratio. Results from cytotoxicity studies showed occurrence of apoptotic cells in the presence of chitosan and ion cross-linked chitosan microparticles, followed by a loss of membrane potential suggesting that cell death might go through the mitochondrial apoptotic pathway.

Ključne besede

hitosan;mikrodelci;citotoksičnost;dostavni sistem;glicerol.2-fosfat;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UL FFA - Fakulteta za farmacijo
UDK: 577.1
COBISS: 4190065 Povezava se bo odprla v novem oknu
ISSN: 1660-3397
Št. ogledov: 167
Št. prenosov: 64
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: hitosan;mikrodelci;citotoksičnost;dostavni sistem;glicerol 2-fosfat;
Vrsta dela (COBISS): Članek v reviji
Strani: 22 str.
Letnik: ǂVol. ǂ14
Zvezek: ǂno. ǂ10
Čas izdaje: 2016
DOI: 10.3390/md14100174
ID: 13576489