Jana Avberšek (Author), Bojan Papić (Author), Darja Kušar (Author), Vladimira Erjavec (Author), Katja Seme (Author), Majda Golob (Author), Irena Zdovc (Author)

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is an important cause of nosocomial infections in humans, but its importance in small animal practice is increasing. Here, we present a case of feline otitis externa (OE) caused by MRSA; both hemolytic and nonhemolytic variants with a stable phenotype were recovered from the external auditory canal after infection was detected by routine otoscopy. One isolate per variant underwent antimicrobial susceptibility testing (AST) by broth microdilution method, conventional spa typing and whole-genome sequencing (WGS). The results showed that both variants were genetically related and were of sequence type (ST) 1327, SCCmec type IV and spa type t005. AST and WGS showed that both isolates were resistant to β-lactams and sensitive to all tested non-β-lactam antibiotics. Both isolates were pvl-negative, but encoded several other virulence genes (aur, hlgABC, sak, scn, seg, sei, sem, sen, seo and seu). Genetic background of the mixed hemolytic phenotype was not identified; no differences in the agr locus or other regulatory regions were detected. Three single-nucleotide polymorphisms were identified but could not be associated with hemolysis. This well-documented case of MRSA infection in companion animals adds to the reports of MRSA infections with a mixed hemolytic phenotype.

Keywords

Veterinarska medicina;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UL VF - Veterinary Faculty
UDC: 636.8.09:579:57.08
COBISS: 64125443 Link will open in a new window
ISSN: 2079-6382
Views: 188
Downloads: 70
Average score: 0 (0 votes)
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Other data

Secondary language: Slovenian
Secondary keywords: Staphylococcus aureus;Genetics;Whole-genome sequencing;Otitis externa;Microbiology;Cats;
Type (COBISS): Article
Pages: art. 599, str. 1-11
Volume: ǂVol. ǂ10
Issue: ǂno. ǂ5
Chronology: 2021
DOI: 10.3390/antibiotics10050599
ID: 13689638
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