Jana Avberšek (Avtor), Bojan Papić (Avtor), Darja Kušar (Avtor), Vladimira Erjavec (Avtor), Katja Seme (Avtor), Majda Golob (Avtor), Irena Zdovc (Avtor)

Povzetek

Methicillin-resistant Staphylococcus aureus (MRSA) is an important cause of nosocomial infections in humans, but its importance in small animal practice is increasing. Here, we present a case of feline otitis externa (OE) caused by MRSA; both hemolytic and nonhemolytic variants with a stable phenotype were recovered from the external auditory canal after infection was detected by routine otoscopy. One isolate per variant underwent antimicrobial susceptibility testing (AST) by broth microdilution method, conventional spa typing and whole-genome sequencing (WGS). The results showed that both variants were genetically related and were of sequence type (ST) 1327, SCCmec type IV and spa type t005. AST and WGS showed that both isolates were resistant to β-lactams and sensitive to all tested non-β-lactam antibiotics. Both isolates were pvl-negative, but encoded several other virulence genes (aur, hlgABC, sak, scn, seg, sei, sem, sen, seo and seu). Genetic background of the mixed hemolytic phenotype was not identified; no differences in the agr locus or other regulatory regions were detected. Three single-nucleotide polymorphisms were identified but could not be associated with hemolysis. This well-documented case of MRSA infection in companion animals adds to the reports of MRSA infections with a mixed hemolytic phenotype.

Ključne besede

Veterinarska medicina;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UL VF - Veterinarska fakulteta
UDK: 636.8.09:579:57.08
COBISS: 64125443 Povezava se bo odprla v novem oknu
ISSN: 2079-6382
Št. ogledov: 188
Št. prenosov: 70
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: Staphylococcus aureus;Genetics;Whole-genome sequencing;Otitis externa;Microbiology;Cats;
Vrsta dela (COBISS): Članek v reviji
Strani: art. 599, str. 1-11
Letnik: ǂVol. ǂ10
Zvezek: ǂno. ǂ5
Čas izdaje: 2021
DOI: 10.3390/antibiotics10050599
ID: 13689638
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