THP-1 cells and pro-inflammatory cytokine productionTHP-1 Cells and pro-inflammatory cytokine production

ǂan ǂin vitro tool for functional characterization of NOD1/NOD2 antagonists

Žiga Jakopin (Author), Emanuela Corsini (Author)

Abstract

THP-1 cells express high levels of native functional nucleotide-binding oligomerization domain 1 (NOD1), NOD2, and Toll-like receptor 4 (TLR4) receptors, and have often been used for investigating the immunomodulatory effects of small molecules. We postulated that they would represent an ideal cell-based model for our study, the aim of which was to develop a new in vitro tool for functional characterization of NOD antagonists. NOD antagonists were initially screened for their effect on NOD agonist-induced interleukin-8 (IL-8) release. Next, we examined the extent to which the selected NOD antagonists block the NOD-TLR4 synergistic crosstalk by measuring the effect of NOD antagonism on tumor necrosis factoralpha - (TNF-alpha) secretion from doubly activated THP- 1 cells. Overall, the results obtained indicate that pro-inflammatory cytokine secretion from THP-1 provides a valuable, simple and reproducible in vitro tool for functional characterization of NOD antagonists.

Keywords

THP-1;cytokines;IL-8;TNF-alpha;immunomodulation;NOD1 antagonist;NOD2 antagonist;functional characterization;

Data

Language:	English
Year of publishing:	2019
Typology:	1.01 - Original Scientific Article
Organization:	UL FFA - Faculty of Pharmacy
UDC:	615.375:61
COBISS:	4770417
ISSN:	1422-0067
Views:	185
Downloads:	63
Average score:	0 (0 votes)
Metadata:

Other data

Secondary language:	Slovenian
Secondary keywords:	Citokini;Imunomodulatorji;
Type (COBISS):	Article
Pages:	str. 1-11
Volume:	ǂVol. ǂ20
Issue:	ǂno. ǂ17
Chronology:	2019
DOI:	10.3390/ijms20174265
ID:	13797502