THP-1 cells and pro-inflammatory cytokine productionTHP-1 Cells and pro-inflammatory cytokine production

ǂan ǂin vitro tool for functional characterization of NOD1/NOD2 antagonists

Žiga Jakopin (Avtor), Emanuela Corsini (Avtor)

Povzetek

THP-1 cells express high levels of native functional nucleotide-binding oligomerization domain 1 (NOD1), NOD2, and Toll-like receptor 4 (TLR4) receptors, and have often been used for investigating the immunomodulatory effects of small molecules. We postulated that they would represent an ideal cell-based model for our study, the aim of which was to develop a new in vitro tool for functional characterization of NOD antagonists. NOD antagonists were initially screened for their effect on NOD agonist-induced interleukin-8 (IL-8) release. Next, we examined the extent to which the selected NOD antagonists block the NOD-TLR4 synergistic crosstalk by measuring the effect of NOD antagonism on tumor necrosis factoralpha - (TNF-alpha) secretion from doubly activated THP- 1 cells. Overall, the results obtained indicate that pro-inflammatory cytokine secretion from THP-1 provides a valuable, simple and reproducible in vitro tool for functional characterization of NOD antagonists.

Ključne besede

THP-1;cytokines;IL-8;TNF-alpha;immunomodulation;NOD1 antagonist;NOD2 antagonist;functional characterization;

Podatki

Jezik:	Angleški jezik
Leto izida:	2019
Tipologija:	1.01 - Izvirni znanstveni članek
Organizacija:	UL FFA - Fakulteta za farmacijo
UDK:	615.375:61
COBISS:	4770417
ISSN:	1422-0067
Št. ogledov:	185
Št. prenosov:	63
Ocena:	0 (0 glasov)
Metapodatki:

Ostali podatki

Sekundarni jezik:	Slovenski jezik
Sekundarne ključne besede:	Citokini;Imunomodulatorji;
Vrsta dela (COBISS):	Članek v reviji
Strani:	str. 1-11
Letnik:	ǂVol. ǂ20
Zvezek:	ǂno. ǂ17
Čas izdaje:	2019
DOI:	10.3390/ijms20174265
ID:	13797502