magistrsko delo
Tamara Savec (Author), Maja Leitgeb (Mentor), Mateja Primožič (Co-mentor)

Abstract

Liposomi so sodobna transportna oblika vnosa prehranskih dopolnil in zdravil, ki se uporabljajo tudi v kozmetiki za dostavljanje učinkovin v različne plasti kože. Zaradi njihove strukture lažje prodrejo v globje plasti kože, kjer s kontroliranim sproščanjem komponent vnesejo v kožo proteine, vitamine, vodo in druge sestavine. Magistrsko delo prikazuje študijo priprave liposomov, ki imajo ustrezne mehanske in kemijske lastnosti za vgrajevanje substanc, ki se uporabljajo v kozmetiki. Obstojnost pripravljenih liposomov smo določili z merjenjem zeta potenciala. Stabilno formulacijo smo dosegli pri vzorcu, katerega smo stresali 2 h in je zeta potencial znašal -38,3 mV. V liposome smo enkapsulirali aktivno učinkovino ciprofloksacin (CIP) v različnih masnih koncentracijah cipr. = (0,006, 0,009, 0,010 in 0,020) mg/mL ter pri različnih časovnih intervalih stresanja tstresanja (enkapsulacije) = (1, 2 in 3) h. Saj je CIP antibiotik druge generacije in se uporablja za zdravljenje različnih bakterijskih okužb, vključno s tistimi, ki jih povzročajo gram-pozitivne in gram-negativne bakterije. Rezultati kažejo, da imajo najvišjo učinkovitost enkapsulacije liposomi, v katere smo enkapsulirali CIP z masno koncentracijo cipr. = 0,010 mg/mL in stresanje tstresanja (enkapsulacije) = 2 h ter najmanjšo standardno napako srednje vrednosti, ki znaša 2,14 %. Učinkovitost enkapsulacije je v tem primeru znašala 91,24 %. Nato smo določali sproščanje ciprofloksacina pri temperaturi 37 °C (telesna temperatura). Ugotovili smo, da količina sproščenega ciprofloksacina strmo narašča prve 3 h, nato s časom hitrost sproščanja upada. Sproščanje ciprofloksacina se ustali po 20 h, kjer smo detektirali 65,2 % sproščenega ciprofloksacina iz liposomov. Z nadaljnjim naraščanjem časa sproščanja ni zaznati signifikantnih razlik v koncentraciji sproščenega ciprofloksacina. Za določitev toplotnih lastnosti liposomov smo izvedli DSC/TGA analizo. Najvišjo temperaturo razpada smo določili vzorcu, kjer so bili liposomi enkapsulirani z masno koncentracijo cipr. = 0,010 mg/mL, tstresanja (enkapsulacie) = 2 h ter so bil skladiščeni teden dni pri 4 °C, kar je enako razpadu čistega ciprofloksacina. Rezultati TGA analize, so pokazali temperaturo razpada liposoma. Liposomom brez enkapsuliranega ciprofloksacina smo analizirali s pomočjo mikroskopa SEM. Iz pridobljenih slik s pomočjo SEM je razvidno, da smo sintetizirali liposome v velikosti med 77,2 nm do 208 nm.

Keywords

ciprofloksacin;enkapsulacija;liposomi;sproščanje aktivne učinkovine;zeta potencial;magistrske naloge;

Data

Language: Slovenian
Year of publishing:
Typology: 2.09 - Master's Thesis
Organization: UM FKKT - Faculty of Chemistry and Chemical Engineering
Publisher: [T. Savec]
UDC: 602.628:615.014.6(043.2)
COBISS: 92299011 Link will open in a new window
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Downloads: 42
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Other data

Secondary language: English
Secondary title: Preparation of liposomes for use in the cosmetics industry
Secondary abstract: Liposomes are a modern transport form for the delivery of food supplements and medicines, which are also used in cosmetics to deliver active ingredients to different layers of the skin. Their shape makes it easier for them to penetrate deeper layers of the skin, where they deliver proteins, vitamins, water and other ingredients into the skin through a controlled release of components. This master thesis presents a study on the preparation of liposomes that have the appropriate mechanical and chemical properties for the incorporation of substances used in cosmetics. The stability of the prepared liposomes was determined by measuring the zeta potential. A stable formulation was achieved for the sample shaken for 2 h at -38.3 mV. The active ingredient ciprofloxacin was encapsulated in liposomes at different mass concentrations cipr. = (0,006, 0,009, 0,010 and 0,020) mg/mL and at different shaking intervals tshaking(encapsulation) = (1, 2 and 3) h. CIP is a second-generation antibiotic and is used to treat a variety of bacterial infections, including those caused by gram-positive and gram-negative bacteria. The results indicate that the highest encapsulation efficiency of liposomes, in which ciprofloxacin was encapsulated with mass concentration of cipr. = 0,010 mg/mL and which was shaken by tshaking(encapsulation) = 2 h and the minimum standard error of the mean value of 2,14 %. Encapsulation efficiency in this case was 91.24 %. These samples were then determined for ciprofloxacin release at 37 °C (body temperature). We found that the amount of ciprofloxacin released increases sharply at the start of the experiment, then the rate of release starts to decrease over time. The release of ciprofloxacin stabilized after 24 h, where 65.2 % of the ciprofloxacin released from liposomes was detected. DSC/TGA analysis was performed to determine the thermal properties of the liposomes. The maximum decay temperature was determined for the sample where the liposomes were encapsulated with mass concentrations cipr. = 0,010 mg/mL, tshaking(encapsulation) = 2 h and were stored for a week at 4 °C, which is equal to the degradation of pure ciprofloxacin. The results of the TGA analysis showed us the decomposition temperature of the liposome. Liposomes without encapsulated ciprofloxacin were analyzed by surface area using an SEM microscope, which showed liposomes that were synthesized between 77.2 nm and 208 nm.
Secondary keywords: ciprofloxacine;encapsulation;liposomes;release of active substance;zeta potential;
Type (COBISS): Master's thesis/paper
Thesis comment: Univ. v Mariboru, Fak. za kemijo in kemijsko tehnologijo
Pages: 1 spletni vir (1 datoteka PDF (XII, 44 f.))
ID: 13886455