Anja Klančnik (Author), Ivana Gobin (Author), Barbara Jeršek (Author), Sonja Smole Možina (Author), Darinka Vučković (Author), Magda Tušek-Žnidarič (Author), Maja Abram (Author)

Abstract

The aim of this study was to evaluate Campylobacter jejuni NTCT 11168 adhesion to abiotic and biotic surfaces when grown in co-culture with Escherichia coli ATCC 11229 and/or Listeria monocytogenes 4b. Adhesion of C. jejuni to polystyrene and to Caco-2 cells and Acanthamoeba castellanii was lower for at least 3 log CFU/mL compared to E. coli and L. monocytogenes. Electron micrographs of ultrathin sections revealed interactions of C. jejuni with host cells. In co-culture with E. coli and L. monocytogenes, adhesion of C. jejuni to all tested surfaces was significantly increased for more than 1 log CFU/mL. There was 10% higher aggregation for C. jejuni than for other pathogens, and high co-aggregation of co-cultures of C. jejuni with E. coli and L. monocytogenes. These data show that C. jejuni in co-cultures with E. coli and L. monocytogenes present significantly higher risk than C. jejuni as mono-cultures, which need to be taken into account in risk evaluation. C. jejuni adhesion is a prerequisite for their colonization, biofilm formation, and further contamination of the environment. C. jejuni survival under adverse conditions as a factor in their pathogenicity and depends on their adhesion to different surfaces, not only as individual strains, but also in co-cultures with other bacteria like E. coli and L. monocytogenes.

Keywords

patogeni mikroorganizmi;Campylobacter jejuni;bakterijska adhezija;bakterijski biofilmi;celična adhezija;polistiren;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UL BF - Biotechnical Faculty
UDC: 579.67:579.26:547.9
COBISS: 5171320 Link will open in a new window
ISSN: 2076-2607
Views: 206
Downloads: 72
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Other data

Secondary language: Slovenian
Secondary keywords: patogeni mikroorganizmi;Campylobacter jejuni;bakterijska adhezija;bakterijski biofilmi;celična adhezija;polistiren;
Type (COBISS): Article
Pages: str. 1-14
Volume: ǂVol. ǂ8
Issue: ǂiss. ǂ2
Chronology: 2020
DOI: 10.3390/microorganisms8020201
ID: 13961679