Anja Klančnik (Avtor), Ivana Gobin (Avtor), Barbara Jeršek (Avtor), Sonja Smole Možina (Avtor), Darinka Vučković (Avtor), Magda Tušek-Žnidarič (Avtor), Maja Abram (Avtor)

Povzetek

The aim of this study was to evaluate Campylobacter jejuni NTCT 11168 adhesion to abiotic and biotic surfaces when grown in co-culture with Escherichia coli ATCC 11229 and/or Listeria monocytogenes 4b. Adhesion of C. jejuni to polystyrene and to Caco-2 cells and Acanthamoeba castellanii was lower for at least 3 log CFU/mL compared to E. coli and L. monocytogenes. Electron micrographs of ultrathin sections revealed interactions of C. jejuni with host cells. In co-culture with E. coli and L. monocytogenes, adhesion of C. jejuni to all tested surfaces was significantly increased for more than 1 log CFU/mL. There was 10% higher aggregation for C. jejuni than for other pathogens, and high co-aggregation of co-cultures of C. jejuni with E. coli and L. monocytogenes. These data show that C. jejuni in co-cultures with E. coli and L. monocytogenes present significantly higher risk than C. jejuni as mono-cultures, which need to be taken into account in risk evaluation. C. jejuni adhesion is a prerequisite for their colonization, biofilm formation, and further contamination of the environment. C. jejuni survival under adverse conditions as a factor in their pathogenicity and depends on their adhesion to different surfaces, not only as individual strains, but also in co-cultures with other bacteria like E. coli and L. monocytogenes.

Ključne besede

patogeni mikroorganizmi;Campylobacter jejuni;bakterijska adhezija;bakterijski biofilmi;celična adhezija;polistiren;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UL BF - Biotehniška fakulteta
UDK: 579.67:579.26:547.9
COBISS: 5171320 Povezava se bo odprla v novem oknu
ISSN: 2076-2607
Št. ogledov: 206
Št. prenosov: 72
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: patogeni mikroorganizmi;Campylobacter jejuni;bakterijska adhezija;bakterijski biofilmi;celična adhezija;polistiren;
Vrsta dela (COBISS): Članek v reviji
Strani: str. 1-14
Letnik: ǂVol. ǂ8
Zvezek: ǂiss. ǂ2
Čas izdaje: 2020
DOI: 10.3390/microorganisms8020201
ID: 13961679