Anastasija Panevska (Author), Gordana Glavan (Author), Anita Jemec (Author), Veronika Kukuljan (Author), Tomaž Trobec (Author), Monika C. Žužek (Author), Milka Vrecl (Author), Damjana Drobne (Author), Robert Frangež (Author), Kristina Sepčić (Author)

Abstract

Aegerolysin proteins ostreolysin A6 (OlyA6), pleurotolysin A2 (PlyA2) and erylysin A (EryA) produced by the mushroom genus Pleurotus bind strongly to an invertebrate-specific membrane sphingolipid, and together with a protein partner pleurotolysin B (PlyB), form transmembrane pore complexes. This pore formation is the basis for the selective insecticidal activity of aegerolysin/PlyB complexes against two economically important coleopteran pests: the Colorado potato beetle and the western corn rootworm. In this study, we evaluated the toxicities of these aegerolysin/PlyB complexes using feeding tests with two ecologically important non-target arthropod species: the woodlouse and the honey bee. The mammalian toxicity of the EryA/PlyB complex was also evaluated after intravenous administration to mice. None of the aegerolysin/PlyB complexes were toxic against woodlice, but OlyA6/PlyB and PlyA2/PlyB were toxic to honeybees, with 48 h mean lethal concentrations (LC$_{50}$) of 0.22 and 0.39 mg/mL, respectively, in their food. EryA/PlyB was also tested intravenously in mice up to 3 mg/kg body mass, without showing toxicity. With no toxicity seen for EryA/PlyB for environmentally beneficial arthropods and mammals at the tested concentrations, these EryA/PlyB complexes are of particular interest for development of new bioinsecticides for control of selected coleopteran pests.

Keywords

stranski učinki;egerolizini;Apis mellifera;bioinsekticidi;erilizin A;protein z domeno MACPF;netarčni organizmi;bukov ostrigar;Porcellio scaber;toksičnost;adverse effects;aegerolysins;bioinsecticides;erylysin A;MACPF protein;non-target organisms;oyster mushroom;toxicity;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UL BF - Biotechnical Faculty
UDC: 577
COBISS: 68710147 Link will open in a new window
ISSN: 2072-6651
Views: 131
Downloads: 39
Average score: 0 (0 votes)
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Other data

Secondary language: Slovenian
Secondary keywords: stranski učinki;egerolizini;Apis mellifera;bioinsekticidi;erilizin A;protein z domeno MACPF;netarčni organizmi;bukov ostrigar;Porcellio scaber;toksičnost;
Type (COBISS): Article
Pages: str. 1-13
Volume: ǂVol. ǂ13
Issue: ǂno. ǂ7
Chronology: 2021
DOI: 10.3390/toxins13070457
ID: 14889979