Matej Milijaš Jotić (Avtor), Anastasija Panevska (Avtor), Ioan Iacovache (Avtor), Rok Kostanjšek (Avtor), Martina Mravinec (Avtor), Matej Skočaj (Avtor), Benoît Zuber (Avtor), Ana Pavšič (Avtor), Jaka Razinger (Avtor), Špela Modic (Avtor), Francesco Trenti (Avtor), Graziano Guella (Avtor), Kristina Sepčić (Avtor)

Povzetek

Ostreolysin A6 (OlyA6) is a protein produced by the oyster mushroom (Pleurotus ostreatus). It binds to membrane sphingomyelin/cholesterol domains, and together with its protein partner, pleurotolysin B (PlyB), it forms 13-meric transmembrane pore complexes. Further, OlyA6 binds 1000 times more strongly to the insect-specific membrane sphingolipid, ceramide phosphoethanolamine (CPE). In concert with PlyB, OlyA6 has potent and selective insecticidal activity against the western corn rootworm. We analysed the histological alterations of the midgut wall columnar epithelium of western corn rootworm larvae fed with OlyA6/PlyB, which showed vacuolisation of the cell cytoplasm, swelling of the apical cell surface into the gut lumen, and delamination of the basal lamina underlying the epithelium. Additionally, cryo-electron microscopy was used to explore the membrane interactions of the OlyA6/PlyB complex using lipid vesicles composed of artificial lipids containing CPE, and western corn rootworm brush border membrane vesicles. Multimeric transmembrane pores were formed in both vesicle preparations, similar to those described for sphingomyelin/cholesterol membranes. These results strongly suggest that the molecular mechanism of insecticidal action of OlyA6/PlyB arises from specific interactions of OlyA6 with CPE, and the consequent formation of transmembrane pores in the insect midgut.

Ključne besede

egerolizin;bioinsekticid;protein z domeno MACPF;bukov ostrigar;porotvorni protein;koruzni hrošč;aegerolysin;bioinsecticide;MACPF-protein;oyster mushroom;pore-forming protein;western corn rootworm;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UL BF - Biotehniška fakulteta
UDK: 577
COBISS: 68691203 Povezava se bo odprla v novem oknu
ISSN: 2072-6651
Št. ogledov: 115
Št. prenosov: 49
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: egerolizin;bioinsekticid;protein z domeno MACPF;bukov ostrigar;porotvorni protein;koruzni hrošč;
Vrsta dela (COBISS): Članek v reviji
Strani: str. 1-16
Letnik: ǂVol. ǂ13
Zvezek: ǂno. ǂ7
Čas izdaje: 2021
DOI: 10.3390/toxins13070455
ID: 14880017