Towards the well-tempered chloroplast DNA sequences

Ante Turudić (Author), Zlatko Liber (Author), Martina Grdiša (Author), Jernej Jakše (Author), Filip Varga (Author), Zlatko Šatović (Author)

Abstract

With the development of next-generation sequencing technology and bioinformatics tools, the process of assembling DNA sequences has become cheaper and easier, especially in the case of much shorter organelle genomes. The number of available DNA sequences of complete chloroplast genomes in public genetic databases is constantly increasing and the data are widely used in plant phylogenetic and biotechnological research. In this work, we investigated possible inconsistencies in the stored form of publicly available chloroplast genome sequence data. The impact of these inconsistencies on the results of the phylogenetic analysis was investigated and the bioinformatic solution to identify and correct inconsistencies was implemented. The whole procedure was demonstrated using five plant families (Apiaceae, Asteraceae, Campanulaceae, Lamiaceae and Rosaceae) as examples.

Keywords

bioinformatika;kloroplasti;genom;sekvenciranje genoma;filogenetske analize;filogenetske metode;

Data

Language:	English
Year of publishing:	2021
Typology:	1.01 - Original Scientific Article
Organization:	UL BF - Biotechnical Faculty
UDC:	602
COBISS:	69203459
ISSN:	2223-7747
Views:	120
Downloads:	46
Average score:	0 (0 votes)
Metadata:

Other data

Secondary language:	Slovenian
Secondary keywords:	bioinformatika;kloroplasti;genom;sekvenciranje genoma;filogenetske analize;filogenetske metode;
Type (COBISS):	Article
Pages:	str. 1-14
Volume:	ǂVol. ǂ10
Issue:	ǂno. ǂ7 (1360)
Chronology:	2021
DOI:	10.3390/plants10071360
ID:	14889985