Nina Warner (Author), Ilja Gasan Osojnik Črnivec (Author), Vijay Kumar Rana (Author), Menandro Cruz (Author), Oren A. Scherman (Author)

Abstract

The encapsulation of proteins into core-shell structures is a widely utilised strategy for controlling protein stability, delivery and release. Despite the recognised utility of these microstructures, however, core-shell fabrication routes are often too costly or poorly scalable to allow for industrial translation. Furthermore, many scalable routes rely upon emulsion-techniques implicating denaturing or environmentally harmful organic solvents. Herein, we investigate core-shell protein encapsulation through single-feed, aqueous spray drying: a cheap, industrially ubiquitous particle-formation technology in the absence of organic solvents. We show that an excipient’s preference for the surface of the spray dried particle is well-predicted by its hydrodynamic diameter (D$_h$) under relevant feed buffer conditions (pH and ionic strength) and that the predictive power of D$_h$ is improved when measured at the spray dryer outlet temperature compared to room temperature (R$^2$ = 0.64 vs. 0.59). Lastly, we leverage these findings to propose an adaptable design framework for fabricating core-shell protein encapsulates by single-feed aqueous spray drying.

Keywords

sušenje z razprševanjem;proteini;kapsulacija;dostavni sistemi;spray dry;protein;encapsulation;formulation;excipient;core-shell;EISA;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UL BF - Biotechnical Faculty
UDC: 66.047:577.112:615.014.6
COBISS: 102605059 Link will open in a new window
ISSN: 1420-3049
Views: 117
Downloads: 66
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Other data

Secondary language: Slovenian
Secondary abstract: The encapsulation of proteins into core-shell structures is a widely utilised strategy for controlling protein stability, delivery and release. Despite the recognised utility of these microstructures, however, core-shell fabrication routes are often too costly or poorly scalable to allow for industrial translation. Furthermore, many scalable routes rely upon emulsion-techniques implicating denaturing or environmentally harmful organic solvents. Herein, we investigate core-shell protein encapsulation through single-feed, aqueous spray drying: a cheap, industrially ubiquitous particle-formation technology in the absence of organic solvents. We show that an excipient’s preference for the surface of the spray dried particle is well-predicted by its hydrodynamic diameter (Dh) under relevant feed buffer conditions (pH and ionic strength) and that the predictive power of Dh is improved when measured at the spray dryer outlet temperature compared to room temperature (R2 = 0.64 vs. 0.59). Lastly, we leverage these findings to propose an adaptable design framework for fabricating core-shell protein encapsulates by single-feed aqueous spray drying.
Secondary keywords: sušenje z razprševanjem;proteini;kapsulacija;dostavni sistemi;
Type (COBISS): Article
Pages: str. 1-20, art. 2197
Volume: ǂVol. ǂ27
Issue: ǂiss. ǂ7
Chronology: 2022
DOI: 10.3390/molecules27072197
ID: 15023655