Nina Warner (Avtor), Ilja Gasan Osojnik Črnivec (Avtor), Vijay Kumar Rana (Avtor), Menandro Cruz (Avtor), Oren A. Scherman (Avtor)

Povzetek

The encapsulation of proteins into core-shell structures is a widely utilised strategy for controlling protein stability, delivery and release. Despite the recognised utility of these microstructures, however, core-shell fabrication routes are often too costly or poorly scalable to allow for industrial translation. Furthermore, many scalable routes rely upon emulsion-techniques implicating denaturing or environmentally harmful organic solvents. Herein, we investigate core-shell protein encapsulation through single-feed, aqueous spray drying: a cheap, industrially ubiquitous particle-formation technology in the absence of organic solvents. We show that an excipient’s preference for the surface of the spray dried particle is well-predicted by its hydrodynamic diameter (D$_h$) under relevant feed buffer conditions (pH and ionic strength) and that the predictive power of D$_h$ is improved when measured at the spray dryer outlet temperature compared to room temperature (R$^2$ = 0.64 vs. 0.59). Lastly, we leverage these findings to propose an adaptable design framework for fabricating core-shell protein encapsulates by single-feed aqueous spray drying.

Ključne besede

sušenje z razprševanjem;proteini;kapsulacija;dostavni sistemi;spray dry;protein;encapsulation;formulation;excipient;core-shell;EISA;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UL BF - Biotehniška fakulteta
UDK: 66.047:577.112:615.014.6
COBISS: 102605059 Povezava se bo odprla v novem oknu
ISSN: 1420-3049
Št. ogledov: 117
Št. prenosov: 66
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarni povzetek: The encapsulation of proteins into core-shell structures is a widely utilised strategy for controlling protein stability, delivery and release. Despite the recognised utility of these microstructures, however, core-shell fabrication routes are often too costly or poorly scalable to allow for industrial translation. Furthermore, many scalable routes rely upon emulsion-techniques implicating denaturing or environmentally harmful organic solvents. Herein, we investigate core-shell protein encapsulation through single-feed, aqueous spray drying: a cheap, industrially ubiquitous particle-formation technology in the absence of organic solvents. We show that an excipient’s preference for the surface of the spray dried particle is well-predicted by its hydrodynamic diameter (Dh) under relevant feed buffer conditions (pH and ionic strength) and that the predictive power of Dh is improved when measured at the spray dryer outlet temperature compared to room temperature (R2 = 0.64 vs. 0.59). Lastly, we leverage these findings to propose an adaptable design framework for fabricating core-shell protein encapsulates by single-feed aqueous spray drying.
Sekundarne ključne besede: sušenje z razprševanjem;proteini;kapsulacija;dostavni sistemi;
Vrsta dela (COBISS): Članek v reviji
Strani: str. 1-20, art. 2197
Letnik: ǂVol. ǂ27
Zvezek: ǂiss. ǂ7
Čas izdaje: 2022
DOI: 10.3390/molecules27072197
ID: 15023655