Detection of herpes simplex and varicella-zoster virus from skin lesions

comparison of RT-PCR and isothermal amplification for rapid identification

Monika Jevšnik (Author), Lara Lusa (Author), Tina Uršič (Author), Urška Glinšek Biškup (Author), Miroslav Petrovec (Author)

Abstract

We compared 2 molecular tests for detection of herpes simplex viruses 1 and 2 (HSV-1, HSV-2) and varicellazoster virus (VZV): real-time polymerase chain reaction (RT-PCR) (Argene, BioMerieux, France) performed on an LC480 platform (Roche Applied Science, Mannheim, Germany) and isothermal amplification using a Solana HSV1 + 2/VZV assay (Quidel Corporation Worldwide Headquarters, San Diego, CA) with helicase-dependent amplification performed by a Solana% instrument. With both methods, HSV-1 was detected in 68/291 (23.4%), HSV-2 in 23/291 (7.9%), and VZV in 48/291 (16.5%) skin lesions. Both methods agreed completely only in detection of HSV-2 (kappa=1). Concordance between Solana HSV1+2/VZV and RT-PCR was 98.3% (kappa=0.95) for HSV-1 and 99.3% (kappa=0.98) for VZV. Rapid detection of HSV-1, HSV-2, and VZV using the Solana platform is a useful method for routine diagnostics and for urgent swab samples requiring a short turnaround time.

Keywords

herpes simplex virus;varicella-zoster virus;skin lesion;

Data

Language:	English
Year of publishing:	2020
Typology:	1.01 - Original Scientific Article
Organization:	UL MF - Faculty of Medicine
UDC:	616.5
COBISS:	34704857
ISSN:	1879-0070
Views:	77
Downloads:	35
Average score:	0 (0 votes)
Metadata:

Other data

Secondary language:	Slovenian
Secondary keywords:	herpes simplex virus;virus varicella-zoster;lezija kože;
Type (COBISS):	Article
Pages:	str. 1-4
Volume:	ǂVol. ǂ 97
Issue:	ǂiss. ǂ2
Chronology:	2020
DOI:	10.1016/j.diagmicrobio.2020.115015
ID:	15786523