comparison of RT-PCR and isothermal amplification for rapid identification
Monika Jevšnik (Avtor), Lara Lusa (Avtor), Tina Uršič (Avtor), Urška Glinšek Biškup (Avtor), Miroslav Petrovec (Avtor)

Povzetek

We compared 2 molecular tests for detection of herpes simplex viruses 1 and 2 (HSV-1, HSV-2) and varicellazoster virus (VZV): real-time polymerase chain reaction (RT-PCR) (Argene, BioMerieux, France) performed on an LC480 platform (Roche Applied Science, Mannheim, Germany) and isothermal amplification using a Solana HSV1 + 2/VZV assay (Quidel Corporation Worldwide Headquarters, San Diego, CA) with helicase-dependent amplification performed by a Solana% instrument. With both methods, HSV-1 was detected in 68/291 (23.4%), HSV-2 in 23/291 (7.9%), and VZV in 48/291 (16.5%) skin lesions. Both methods agreed completely only in detection of HSV-2 (kappa=1). Concordance between Solana HSV1+2/VZV and RT-PCR was 98.3% (kappa=0.95) for HSV-1 and 99.3% (kappa=0.98) for VZV. Rapid detection of HSV-1, HSV-2, and VZV using the Solana platform is a useful method for routine diagnostics and for urgent swab samples requiring a short turnaround time.

Ključne besede

herpes simplex virus;varicella-zoster virus;skin lesion;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UL MF - Medicinska fakulteta
UDK: 616.5
COBISS: 34704857 Povezava se bo odprla v novem oknu
ISSN: 1879-0070
Št. ogledov: 77
Št. prenosov: 35
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: herpes simplex virus;virus varicella-zoster;lezija kože;
Vrsta dela (COBISS): Članek v reviji
Strani: str. 1-4
Letnik: ǂVol. ǂ 97
Zvezek: ǂiss. ǂ2
Čas izdaje: 2020
DOI: 10.1016/j.diagmicrobio.2020.115015
ID: 15786523