diplomsko delo
Pia Kokelj (Author), Uroš Petrovič (Mentor)

Abstract

Celične tovarne se vedno znova izkazujejo ne le za koristen, ampak pogosto tudi bistveni del najrazličnejših vej industrije. Njihova uporabnost sega tako na področje medicine in farmacije, zelene energije, kot izdelave mnogih drugih produktov z visoko dodano vrednostjo. Sevi, ki jih najdemo v naravi v veliki večini primerov sami po sebi ne zadostujejo za izdelavo želenih produktov, zato je najpomembnejši korak pri izdelavi celičnih tovarn uporaba natančnih, zanesljivih in prilagodljivih metod za urejanje genoma izbranega producentskega organizma. Eden izmed takih organizmov je kvasovka Saccharomyces cerevisiae, ki zaradi svoje dovzetnosti za spreminjanje genoma predstavlja uporaben modelni organizem in producenta na industrijski ravni. Pri izdelavi celičnih tovarn si pogosto želimo vpeljati večje število sprememb v genom hkrati, kjer še posebno prav pridejo metode za obsežnejše urejanje genoma. Metode, ki temeljijo na CRISPR/Cas, v takih primerih trenutno zasedajo prvo mesto, a nam še vseeno ne dopuščajo neomejene širine in globine, ko pride do bolj zapletenih in celovitih urejanj genoma. Če stopimo korak dlje in pogledamo na izdelavo celičnih tovarn z vidika ustvarjanja in ne le urejanja že obstoječega genoma, se pri njegovi popolni de novo sintezi zaenkrat srečamo z le nekaterimi uspešnimi primeri pri manjših genomih, ter posledično več težavami kot rešitvami pri uporabi te tehnologije v industrijske namene. V skladu s tem je trenutno najboljša izbira pri izdelavi kvalitetnih in ekonomičnih celičnih tovarn povezava de novo sinteze zapisa DNA z metodami za obsežnejše urejanje genoma.

Keywords

biotehnologija;CRISPR/Cas;Saccharomyces cerevisiae;sintezna biologija;celične tovarne;

Data

Language: Slovenian
Year of publishing:
Typology: 2.11 - Undergraduate Thesis
Organization: UL BF - Biotechnical Faculty
Publisher: [P. Kokelj]
UDC: 601.4:577.21:575.113:576(043.2)
COBISS: 114891779 Link will open in a new window
Views: 80
Downloads: 27
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Other data

Secondary language: English
Secondary title: Use of CRISPR/Cas to modify the genome of Saccharomyces cerevisiae in the creation of cell factories
Secondary abstract: Time and again, cell factories are proving to be not only a useful, but often an essential part of a wide range of industries. Their applications range from medicine and pharmaceuticals, to green energy and the manufacture of many other high value-added products. In the vast majority of cases, the strains found in nature are not sufficient in producing the desired product on their own, so the most important step in the construction of cell factories is to use precise, reliable and scalable methods to edit the genome of the selected producer organism. One such organism is the yeast Saccharomyces cerevisiae, which, due to its susceptibility to genome editing, represents a useful model organism and producer at an industrial level. In the construction of cell factories, it is often desirable to introduce a large number of changes to the genome at the same time, where large-scale genome editing methods are particularly useful. CRISPR/Cas-based methods are currently the first choice in such cases, but still do not allow for an unlimited breadth and depth when it comes to more complex and comprehensive genome editing. Taking a step further and looking at the construction of cell factories from the point of view of creating, rather than just editing an existing genome, we have so far been confronted with only a few successful examples of complete de novo synthesis of smaller genomes, and consequently more problems than solutions when it comes to the use of this technology for industrial purposes. Accordingly, the best choice for the production of high quality and economical cell factories at present is to combine de novo DNA synthesis and large-scale genome editing methods.
Secondary keywords: biotechnology;synthetic biology;cell factories;
Type (COBISS): Bachelor thesis/paper
Study programme: 0
Thesis comment: Univ. v Ljubljani, Biotehniška fak., Študij biotehnologije
Pages: VI, 21 str.
ID: 15891142