diplomsko delo
Matija Hrovatin (Avtor), Uroš Petrovič (Mentor), Uroš Petrovič (Član komisije za zagovor), Polona Jamnik (Član komisije za zagovor), Mojca Narat (Član komisije za zagovor)

Povzetek

Za proizvodnjo širokega spektra produktov se uporabljajo celične tovarne, ki lahko kot vir energije uporabljajo obnovljive vire in odpadke. Optimizacija delovanja celičnih tovarn je možna z metabolnim inženiringom. Ena od metod je sistem CRISPR-Cas9, ki učinkovito uvede želene spremembe v genom tarčnega organizma. Običajno s sistemom CRISPR-Cas9 izvedemo delecije, insercije ter spremembe tarčnih genov tako, da povečamo metabolni pretok skozi tarčne metabolne poti. Dobimo učinkovito celično tovarno, ki proizvaja velike količine tarčnega produkta. Tehnologijo CRISPR-Cas9 odlikuje visoka natančnost uvedbe sprememb, enostavnost, hitrost in nizka cena. V diplomski nalogi sem opisal aktualne uporabe tehnologije CRISPR-Cas9 za namen metabolnega inženiringa in izdelavo učinkovitih sevov mikroorganizmov, ki imajo velik potencial za uporabo v živilski, kozmetični in kemijski industriji ter v proizvodnji zdravil in biogoriv. Metabolni inženiring je ključnega pomena za nadaljnji razvoj in proizvodnjo produktov s celičnimi tovarnami.

Ključne besede

metabolni inženiring;CRISPR-Cas9;celične tovarne;preurejanje genoma;

Podatki

Jezik: Slovenski jezik
Leto izida:
Tipologija: 2.11 - Diplomsko delo
Organizacija: UL BF - Biotehniška fakulteta
Založnik: [M. Hrovatin]
UDK: 606:62:602.6:578/579:601.4:577.21(043.2)
COBISS: 31219715 Povezava se bo odprla v novem oknu
Št. ogledov: 368
Št. prenosov: 90
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Angleški jezik
Sekundarni naslov: Use of CRISPR-Cas9 technology in metabolic engineering
Sekundarni povzetek: Cell factories are applicable for production of a wide range of products from renewable sources and waste materials as an energy source. Optimization of cell factories operation conditions is further possible with metabolic engineering. One of the methods to achieve this is the CRISPR-Cas9 technology, which effectively introduces the desired changes to the genome of the target organism. Typically, deletions, insertions and other manipulations of target genes are performed with the CRISPR-Cas9 with the aim to increase metabolic flux through the target metabolic pathways. Using this approach, large quantities of the target product is synthesised through an environmentally benign process, via efficient cell factories. The advantages of CRISPR-Cas9 are high precision of genetic changes at targeted loci, simplicity, speed and economic efficiency of the process. In this thesis I will describe the state-of-the-art applications of CRISPR-Cas9 technology in metabolic engineering. I will also address production of efficient cell lines with potential applications in various fields such as food, cosmetic and chemical industry, production of biofuels and pharmaceuticals. Metabolic engineering is a key factor for the further development and manufacturing of desired products with cell factories.
Sekundarne ključne besede: metabolic engineering;cell factories;genome modification;
Vrsta dela (COBISS): Diplomsko delo/naloga
Študijski program: 0
Konec prepovedi (OpenAIRE): 1970-01-01
Komentar na gradivo: Univ. v Ljubljani, Biotehniška fak., Študij biotehnologije
Strani: VI, 20 str.
ID: 12029447