Contactless delivery of plasmid encoding EGFP in vivo by high-intensity pulsed electromagnetic field

Matej Kranjc (Author), Simona Kranjc (Author), Gregor Serša (Author), Damijan Miklavčič (Author)

Abstract

High-Intensity Pulsed Electromagnetic Fields (HI-PEMF) treatment is an emerging noninvasive and contactless alternative to conventional electroporation, since the electric field inside the tissue is induced remotely by external pulsed magnetic field. Recently, HI-PEMF was applied for delivering siRNA molecules to silence enhanced green fluorescent protein (EGFP) in tumors in vivo. Still, delivered siRNA molecules were 21 base pairs long, which is 200-times smaller compared to nucleic acids such as plasmid DNA (pDNA) that are delivered in gene therapies to various targets to generate therapeutic effect. In our study, we demonstrate the use HI-PEMF treatment as a feasible noninvasive approach to achieve in vivo transfection by enabling the transport of larger molecules such as pDNA encoding EGFP into muscle and skin. We obtained a long-term expression of EGFP in the muscle and skin after HI-PEMF, in some mice even up to 230 days and up to 190 days, respectively. Histological analysis showed significantly less infiltration of inflammatory mononuclear cells in muscle tissue after the delivery of pEGFP using HI-PEMF compared to conventional gene electrotransfer. Furthermore, the antitumor effectiveness using HI-PEMF for electrotransfer of therapeutic plasmid, i.e., silencing MCAM was demonstrated. In conclusion, feasibility of HI-PEMF was demonstrated for transfection of different tissues (muscle, skin, tumor) and could have great potential in gene therapy and in DNA vaccination.

Keywords

magnetno polje;električno polje;permeabilizacija membrane;elektroporacija;zeleni fluorescenčni protein;magnetic fields;electric fields;membrane permeabilization;electroporation;enhanced green fluorescent protein;

Data

Language:	English
Year of publishing:	2021
Typology:	1.01 - Original Scientific Article
Organization:	UL FE - Faculty of Electrical Engineering
UDC:	602.621
COBISS:	64622083
ISSN:	1567-5394
Views:	87
Downloads:	19
Average score:	0 (0 votes)
Metadata:

Other data

Secondary language:	Slovenian
Secondary keywords:	magnetno polje;električno polje;permeabilizacija membrane;elektroporacija;zeleni fluorescenčni protein;
Type (COBISS):	Article
Pages:	str. 1-10
Volume:	ǂVol. ǂ141
Issue:	107847
Chronology:	Oct. 2021
DOI:	10.1016/j.bioelechem.2021.107847
ID:	16120574