Damijan Miklavčič (Avtor), Vitalij Novickij (Avtor), Matej Kranjc (Avtor), Tamara Polajžer (Avtor), Saša Haberl (Avtor), Tina Batista Napotnik (Avtor), Rok Romih (Avtor), Darja Lisjak (Avtor)

Povzetek

Pulsed electric fields (PEFs) can be used to transiently increase cell membrane permeability in procedures ranging from gene therapy to tumor eradication. Although very efficient, PEF-based therapies generally require the use of invasive electrodes, which cause pain and tissue damage. An emerging noninvasive, contactless alternative to PEFs are High Intensity Pulsed Electromagnetic Fields (HI-PEMF), whereby the electric field inside the tissue is induced remotely by external pulsed magnetic field. However, one of the current major drawbacks of HI-PEMFs is their inferior efficiency compared to PEFs. In this study we present the proof-of-concept that by adding highly conductive 5 and 20 nm gold nanoparticles (Au NPs), we can significantly potentiate the permeabilizing effect of HI-PEMFs, making it possible to permeabilize up to 80% of the cells with minimal or no effect on cell survival, compared to negligible percentage of permeabilized cells using HI-PEMF alone. Experiments, conducted on Chinese Hamster Ovary cells and Escherichia coli, suggest that Au NPs act as distributed nanoelectrodes, locally enhancing the electric field induced at the plasma membrane. Our findings open up an avenue of possibilities for combining naked as well as functionalized Au NPs with HI-PEMFs for noninvasive, remotely controlled smart drug delivery applications.

Ključne besede

magnetno polje;permeabilizacija membrane;zlati nanodelci;neinvazivna elektroporacija;nanodelci;magnetic fields;membrane permeabilization;gold nanoparticles;non-invasive electroporation;nanoparticles;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UL FE - Fakulteta za elektrotehniko
UDK: 602.621
COBISS: 32990759 Povezava se bo odprla v novem oknu
ISSN: 1567-5394
Št. ogledov: 538
Št. prenosov: 149
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: magnetno polje;permeabilizacija membrane;zlati nanodelci;neinvazivna elektroporacija;nanodelci;
Vrsta dela (COBISS): Članek v reviji
Strani: str. 107440-1-107440-9
Zvezek: ǂVol. ǂ132
Čas izdaje: Apr. 2020
DOI: 10.1016/j.bioelechem.2019.107440
ID: 13163787