Določanje vitaminov B v prehranskih dopolnilih

diplomsko delo

Luka Racman Knez (Author), Helena Prosen (Mentor)

Abstract

V diplomskem delu sem razvil in delno validiral HPLC metodo za določanje pet vitaminov skupine B v prehranskih dopolnilih. Delo je obsegalo pripravo ustreznih standardnih raztopin, optimizacijo pogojev analizne metode, pripravo umeritvenih premic, delno validacijo metode, pripravo in analizo vzorcev. Metodo mi je uspelo optimizirati do te mere, da lahko z njo kvalitativno in kvantitativno določim vsebnost petih vitaminov skupine B v enostavnih vodotopnih vzorcih. Delna validacija je obsegala določitev linearnosti, ponovljivosti, meje zaznave in meje kvantifikacije. Metodo lahko uporabimo za analize vzorcev, ki vsebujejo niacin (vitamin B3), pantotensko kislino (vitamin B5), piridoksin (vitamin B6), biotin (vitamin B7), in cianokobalamin (vitamin B12). Metodo sem preizkusil z analizo dveh vzorcev, in sicer sem določil vitamine v vitaminskem instant napitku Siti z okusom pomaranče ter v CBD vodotopnih kapljicah z vitamin B kompleksom.

Keywords

prehranska dopolnila;vitamini B;HPLC;validacija metode;diplomska dela;

Data

Language:	Slovenian
Year of publishing:	2022
Typology:	2.11 - Undergraduate Thesis
Organization:	UL FKKT - Faculty of Chemistry and Chemical Technology
Publisher:	[L. Racman Knez]
UDC:	543.544.5HPLC(043.2)
COBISS:	120713731
Views:	52
Downloads:	23
Average score:	0 (0 votes)
Metadata:

Other data

Secondary language:	English
Secondary title:	Determination of B vitamins in food supplements
Secondary abstract:	In my thesis, I developed and partially validated an HPLC method for the determination of five B vitamins in food supplements. The work included preparation of appropriate standard solutions, optimization of the analytical method conditions, preparation of calibration curves, partial validation of the method, sample preparation and analysis. I have managed to optimize the method to the extent that it can be used to qualitatively and quantitatively determine the content of five B vitamins in simple watersoluble samples. The partial validation included determination of linearity, repeatability, limit of detection and limit of quantification. The method can be applied to the analysis of samples containing niacin (vitamin B3), pantothenic acid (vitamin B5), pyridoxine (vitamin B6), biotin (vitamin B7), and cyanocobalamin (vitamin B12). I have tested the method by analysing two samples. I determined the vitamins in Siti vitamin instant drink with orange flavour and in CBD water-soluble drops with vitamin B complex.
Secondary keywords:	B vitamins;liquid chromatography;food supplements;validation;Tekočinska kromatografija;Univerzitetna in visokošolska dela;
Type (COBISS):	Bachelor thesis/paper
Study programme:	1000374
Embargo end date (OpenAIRE):	1970-01-01
Thesis comment:	Univ. v Ljubljani, Fak. za kemijo in kemijsko tehnologijo, VSŠ Kemijska tehnologija
Pages:	36 str.
ID:	16270675