diplomsko delo
Zala Vraničar (Author), Bojana Bogovič Matijašić (Mentor)

Abstract

Mlečnokislinske bakterije so sposobne proizvajati številne pomembne metabolite, med katerimi so tudi bakteriocini. Bakteriocini so peptidi s protimikrobnim delovanjem, ki so že dolgo v uporabi v živilstvu, v zadnjem času pa se njihova uporaba širi tudi na farmacevtsko in medicinsko področje, bodisi kot alternativa za protibakterijska sredstva ali kot pomožna sredstva pri zdravljenju raka. Ker je rak ena izmed najtežjih in najbolj razširjenih bolezni, sodobne terapije pa imajo še vedno preveč stranskih učinkov, obstaja potreba po možnostih zdravljenja z novimi pristopi. Bakteriocini mlečnokislinskih bakterij, kot so nizin, enterocin in plantaricin, so se izkazali kot možni kandidati za pomoč pri zdravljenju različnih oblik raka, saj inhibirajo rast rakavih celic in v njih inducirajo apoptozo, medtem ko nimajo citotoksičnega učinka na zdrave celice. Med možnimi načini delovanja omenjajo povečanje pretočnosti celičnih membran in tvorbo ionskih kanalov na membranah rakavih celic, kar poveča sproščanje laktat dehidrogenaze. Hkrati lahko bakteriocini spodbujajo kopičenje reaktivnih kisikovih radikalov in povečajo apoptotični indeks. Poleg tega lahko zavirajo mitohondrijski energijski metabolizem in glikolizo ter migracijo in proliferacijo rakavih celic. Študije bakteriocinov so trenutno omejene le na raziskave in vitro in na miših, tako da bodo v prihodnosti potrebne tudi dobro nadzorovane klinične raziskave. Odpirajo se tudi možnosti izboljšanja lastnosti naravno prisotnih bakteriocinov z bioinženiringom ali sinergijskimi kombinacijami bakteriocinov z drugimi učinkovinami, ki bi izboljšale obstojnost ter učinkovitost bakteriocinov.

Keywords

biotehnologija;bakteriocini;mlečnokislinske bakterije;zdravljenje;karcinom;nizin;lantibiotik;

Data

Language: Slovenian
Year of publishing:
Typology: 2.11 - Undergraduate Thesis
Organization: UL BF - Biotechnical Faculty
Publisher: [Z. Vraničar]
UDC: 602.3:579.864:604.4:615.277.3(043.2)
COBISS: 119800067 Link will open in a new window
Views: 100
Downloads: 10
Average score: 0 (0 votes)
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Other data

Secondary language: English
Secondary title: Use of bacteriocins of lactic acid bacteria in the cancer treatment
Secondary abstract: Lactic acid bacteria are able to produce a number of important metabolites, including bacteriocins. Bacteriocins are peptides with antimicrobial activity that have long been used in food production, but their use has recently expanded into the pharmaceutical and medical field, either as alternatives to antibacterial agents or as adjuvants in cancer treatment. As cancer is one of the most difficult and widespread diseases and modern therapies stil have too many side effects, there is a need for treatment options with new approaches. Bacteriocins from lactic acid bacteria such as nisin, enterocin and plantaricin have emerged as potential candidates to help treat various cancers, as they inhibit the growth of cancer cells and induce apoptosis in them, while having no cytotoxic effect on healthy cells. Possible modes of action include increasing the fluidity of cell membranes and the formation of ion channels on cancer cell membranes, which increases the release of lactate dehydrogenase. At the same time bacteriocins can promote the accumulation of reactive oxygen radicals and increase the apoptotic index. In addition, they can inhibit mitochondrial energy metabolism and glycolysis, as well as cancer cell migration and proliferation. Studies on bacteriocins are currently limited to in vitro and mouse studies, so well-controlled clinical studies will be needed in the future. There are also possibilities to improve the properties of naturally ocurring bacteriocins through bioengineering or synergistic combinations of bacteriocins with other active ingredients to improve the stability and efficacy od bacteriocins.
Secondary keywords: biotechnology;bacteriocins;lactic acid bacteria;cancer treatment;nisin;lantibiotic;
Type (COBISS): Bachelor thesis/paper
Study programme: 0
Embargo end date (OpenAIRE): 1970-01-01
Thesis comment: Univ. v Ljubljani, Biotehniška fak., Študij biotehnologije
Pages: 1 spletni vir (1 datoteka PDF (VI, 19 f.))
ID: 16306616