Vita Rozman (Avtor), Petra Mohar Lorbeg (Avtor), Primož Treven (Avtor), Tomaž Accetto (Avtor), Sandra Janežič (Avtor), Maja Rupnik (Avtor), Bojana Bogovič Matijašić (Avtor)

Povzetek

Lactic acid bacteria (LAB) and Bifidobacterium sp. (bifidobacteria) can carry antimicrobial resistance genes (ARGs), yet data on resistance mechanisms in these bacteria are limited. The aim of our study was to identify the underlying genetic mechanisms of phenotypic resistance in 103 LAB and bifidobacteria using whole-genome sequencing. Sequencing data not only confirmed the presence of 36 acquired ARGs in genomes of 18 strains, but also revealed wide dissemination of intrinsic ARGs. The presence of acquired ARGs on known and novel mobile genetic elements raises the possibility of their horizontal spread. In addition, our data suggest that mutations may be a common mechanism of resistance. Several novel candidate resistance mechanisms were uncovered, providing a basis for further in vitro studies. Overall, 1,314 minimum inhibitory concentrations matched with genotypes in 92.4% of the cases; however, prediction of phenotype based on genotypic data was only partially efficient, especially with respect to aminoglycosides and chloramphenicol. Our study sheds light on resistance mechanisms and their transferability potential in LAB and bifidobacteria, which will be useful for risk assessment analysis.

Ključne besede

mikorbiologija;bakterije;mlečnokislinske bakterije;genetika;genomika;odpornost;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UL BF - Biotehniška fakulteta
Založnik: Life Science Alliance, LLC
UDK: 579:575
COBISS: 141893123 Povezava se bo odprla v novem oknu
ISSN: 2575-1077
Št. ogledov: 20
Št. prenosov: 0
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: mikorbiologija;bakterije;mlečnokislinske bakterije;genetika;genomika;odpornost;
Vrsta dela (COBISS): Znanstveno delo
Strani: str. 1-15
Letnik: ǂVol. ǂ6
Zvezek: ǂno. ǂ4, [aricle no.] 202201637
Čas izdaje: 2023
DOI: 10.26508/lsa.202201637
ID: 18434266