Vita Rozman (Avtor), Petra Mohar Lorbeg (Avtor), Primož Treven (Avtor), Tomaž Accetto (Avtor), Majda Golob (Avtor), Irena Zdovc (Avtor), Bojana Bogovič Matijašić (Avtor)

Povzetek

Lactic acid bacteria (LAB) and bifidobacteria may serve as reservoirs of antimicrobial resistance, but the risk posed by strains intentionally introduced into the agro-food chain has not yet been thoroughly investigated. The aim of our study was to evaluate whether probiotics, starter and protective cultures, and feed additives represent a risk to human health. In addition to commercial strains of LAB and bifidobacteria, isolates from human milk or colostrum, intestinal mucosa or feces, and fermented products were analyzed. Phenotypic susceptibility data of 474 strains showed that antimicrobial resistance was more common in intestinal isolates than in commercial strains. Antimicrobial resistance genes (ARGs) and mobile genetic elements (MGEs) were characterized in the whole genome sequences of 1114 strains using comparative genomics. Intrinsic ARGs were abundant in enterococci, bifidobacteria, and lactococci but were considered non-risky due to the absence of MGEs. The results revealed that 13.8% of commercial strains contained acquired ARGs, most frequently for tetracycline. We associated 75.5% of the acquired ARGs with known or novel MGEs, and their potential for transmission was assessed by examining metagenomic sequences. We confirmed that ARGs and MGEs were not as abundant or diverse in commercial strains as in human intestinal isolates or isolates from human milk, suggesting that strains intentionally introduced into the agro-food chain do not pose a significant threat. However, attention should be paid especially to individual probiotic strains containing elements that have been shown to have high potential for transferability in the gut microbiota.

Ključne besede

mikrobiologija;mlečnokislinske bakterije;bifidobakterije;agroživilska veriga;odpornost;genetika;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UL BF - Biotehniška fakulteta
UDK: 579:575
COBISS: 123511555 Povezava se bo odprla v novem oknu
ISSN: 1949-0984
Št. ogledov: 349
Št. prenosov: 94
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: mikrobiologija;mlečnokislinske bakterije;bifidobakterije;agroživilska veriga;odpornost;genetika;
Vrsta dela (COBISS): Članek v reviji
Strani: str. 1-17
Letnik: ǂno. ǂ1
Zvezek: ǂart. ǂ2127438
Čas izdaje: 2022
DOI: 10.1080/19490976.2022.2127438
ID: 16716423