magistrsko delo
Janja Pukmajster (Avtor), Nataša Fidler Mis (Recenzent), Bojana Bogovič Matijašić (Mentor), Petra Mohar Lorbeg (Komentor)

Povzetek

Namen magistrske naloge je bil raziskati, ali mlečnokislinske bakterije, predvsem laktobacili, in bifidobakterije iz humanega mleka, kolostruma in zrelega mleka, vsebujejo gene za odpornost proti tetraciklinu in eritromicinu, kar predstavlja določeno tveganje za prenos odpornosti na patogene bakterije v dojenčkovem črevesju. Odpornost proti kanamicinu smo priključili iz razloga, ker je bila v predhodnih raziskavah teh sevov nepričakovano pogosta. Tako smo raziskali, ali so v njihovem genomu prisotni pridobljeni geni z zapisi za odpornost proti izbranim antibiotikom. En vzorec kolostruma in 38 vzorcev mleka smo nacepili na gojišči Rogosa in WCA-mup ter osamili 243 izolatov. Rezultati sekvenciranja izolirane DNA so pokazali, da smo s teh gojišč osamili bakterije iz rodov Lactobacillus, Propionibacterium, Streptococcus, Bifidobacterium in Actinomyces. Tem izolatom smo priključili še 56 predhodno izoliranih bakterijskih sevov iz rodov Lactobacillus in Bifidobacterium. Fenotipsko odpornost smo ugotavljali s pomočjo mikrodilucije. Pri sevih, ki so izkazovali fenotipsko odpornost proti določenemu antibiotiku, smo z uporabo PCR metode preverjali prisotnost najbolj znanih genov, povezanih z odpornostjo proti temu antibiotiku. Fenotipsko odpornost proti eritromicinu smo zaznali pri 3 sevih, proti tetraciklinu pri 10 sevih in proti kanamicinu pri 52 sevih, medtem ko prisotnosti genov erm(A), erm(B), erm(C), erm(T), tet(A), tet(B), tet(C), tet(M), tet(O), tet(W), aac(6’)-aph(2’’), ant(6)-Ia in aph(3’)-IIIa nismo potrdili pri nobenem od teh sevov.

Ključne besede

humano mleko;mlečnokislinske bakterije;bifidobakterije;odpornost proti antibiotikom;eritromicin;tetraciklin;geni za odpornost;

Podatki

Jezik: Slovenski jezik
Leto izida:
Tipologija: 2.09 - Magistrsko delo
Organizacija: UL BF - Biotehniška fakulteta
Založnik: [J. Pukmajster]
UDK: 579.24/.26:612.664:615.33
COBISS: 4858488 Povezava se bo odprla v novem oknu
Št. ogledov: 1481
Št. prenosov: 559
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Angleški jezik
Sekundarni naslov: ǂThe ǂresistance of lactic acid bacteria and bifidobacteria from human colostrum and milk to tetracycline and erythromycin
Sekundarni povzetek: The aim of this master's thesis was to investigate whether lactic acid bacteria, especially lactobacilli, and bifidobacteria from human milk, colostrum and mature milk, contain tetracycline and erythromycin resistance genes, which presents a certain risk of spread of antibiotic resistance to pathogenic bacteria in the infant's gut. Kanamycin was subsequently added to the study for a reason that in previous studies the resistance of lactobacili to this antibiotic was unexpectedly frequent. Thus, we investigated whether the genes associated with the resistance to selected antibiotics are present in the genome of lactic acid bacteria and bifidobacteria. One sample of colostrum and 38 samples of milk were plated on Rogosa and WCA-mup medium. 243 bacterial strains were isolated. The results of the sequencing of isolated DNA showed that bacteria belonged to the genera Lactobacillus, Propionibacterium, Streptococcus, Bifidobacterium and Actinomyces. We also included 56 pre-isolated bacterial strains from the genera Lactobacillus and Bifidobacterium. Phenotypic resistance was determined by microdilution. In phenotypic resistant strains we investigated the presence of the most well-known genes associated with resistance to a particular antibiotic with PCR. Phenotypic resistance to erythromycin was detected in 3 strains, tetracycline in 10 strains, and kanamycin in 52 strains, while the presence of investigated genes erm(A), erm(B), erm(C), erm(T), tet(A), tet(B), tet(C), tet(M), tet(O), tet(W), aac(6')-aph(2''), ant(6)-Ia and aph(3')-IIIa has not been confirmed in any of the strains.
Sekundarne ključne besede: human milk;lactic acid bacteria;Bifidobacterium;antibiotic resistance;erythromycin;tetracycline;antibiotic resistance genes;
Vrsta dela (COBISS): Magistrsko delo/naloga
Študijski program: 0
Komentar na gradivo: Univ. v Ljubljani, Biotehniška fak., Oddelek za živilstvo
Strani: IX, 45 f.
ID: 10914891