diplomsko delo
Stefanija Ivanova (Author), Ksenija Kogej (Mentor)

Abstract

Liposomi so vezikularni strukturi, ki so sposobni ujeti tako hidrofobnih kot hidrofilnih spojin in se zato lahko uporabljajo kot nosilci za dostavo zdravil in makromolekul v človeška in živalska telesa. Liposome lahko izdelamo iz naravnih in sintetičnih fosfolipidov. V diplomskem delu smo se ukvarjali z novo liposomsko formulacijo brez uporabe farmakoloških neželenih organskih topil, ki so škodljivi za okolje, pri pripravi, uporabili smo dva različna lipida: jajčni lecitin in tekoči sončnični lecitin ter mešanice glicerola in vode kot topila za lipide v razližnih utežnih razmerjih. Raziskali smo velikost veziklov v pripravljenih suspenzijah lecitin/glicerol/voda z metodo dinamičnega sipanja svetlobe. Zanimal nas je še vpliv temperature na hidrodinamski radij veziklov ter ali dodatek olja CBD oziroma vodne raztopine lizocima vplivata na velikost veziklov. Potrdili smo hipotezo, da je velikost veziklov odvisna od razmerja med lecitinom, glicerolom in vodo. Več kot je v suspenziji glicerola, večji so vezikli. Ob dodatku olja CBD nastanejo manjši vezikli, v prisotnosti lizocima pa večji.

Keywords

lecitin;CBD olje;lizocim;hidrodinamski radij;stabilnost;diplomska dela;

Data

Language: Slovenian
Year of publishing:
Typology: 2.11 - Undergraduate Thesis
Organization: UL FKKT - Faculty of Chemistry and Chemical Technology
Publisher: [S. Ivanova]
UDC: 544.7(043.2)
COBISS: 127229955 Link will open in a new window
Views: 33
Downloads: 13
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Other data

Secondary language: English
Secondary title: Stability of vesicular structures in lipid/glycerol/water mixtures
Secondary abstract: Liposomes are vesicular structures capable of entrapping both hydrophobic and hydrophilic compounds and can therefore be used as carriers for the delivery of drugs and macromolecules into human and animal bodies. Liposomes can be made from natural and synthetic phospholipids. In this study, we dealt with a new liposomal formulation without the use of pharmacologically unwanted organic solvents that are harmful to the environment in the preparation, we used two different lipids: egg lecithin and liquid sunflower lecithin, and mixtures of glycerol and water as lipid solvents in different weight ratios. We investigated the size of the vesicles in the prepared lecithin/glycerol/water suspensions using the dynamic light scattering method. We were interested in the effect of temperature on the hydrodynamic radius of the vesicles and whether the addition of CBD oil or the aqueous solution of lysozyme affects the size of the vesicles. We confirmed the hypothesis that the size of the vesicles depends on the ratio between lecithin, glycerol and water. If there is more glycerol in the suspension, larger vesicles form. Smaller vesicles form when CBD oil is added, however larger ones do so when lysozyme is present.
Secondary keywords: liposomes;lecithin;CBD oil;lysozyme;hydrodynamic radius;stability;Liposomi;Univerzitetna in visokošolska dela;
Type (COBISS): Bachelor thesis/paper
Study programme: 1000371
Embargo end date (OpenAIRE): 1970-01-01
Thesis comment: Univ. v Ljubljani, Fak. za kemijo in kemijsko tehnologijo, UNI Biokemija
Pages: 46 str.
ID: 16391548