diplomsko delo
Matic Kink (Author), Davor Kržišnik (Mentor), Miha Humar (Co-mentor)

Abstract

Onesnaženje s sintetičnimi polimeri (plastiko) predstavlja enega večjih problemov našega časa. Plastika je bila najdena skoraj povsod po svetu, od Marianskega jarka do Mount Eversta. Ima negativni vpliv na ekosistem, saj zaradi plastičnih odpadkov umirajo živali. Dokazano je, da je plastika prisotna tudi v človeških telesih. Eden najpogosteje uporabljenih polimerov je polietilen nizke gostote (LDPE) V diplomski nalogi smo poskušali najti boljši postopek za razgradnjo LDPE z glivama bele trohnobe. Uporabili smo glivi Pleurotus ostreatus in Ceriporiopsis subvermispora. Postavili smo hipotezo, da glivi hitreje razgrajata plastiko ob prisotnosti lignina v gojišču. Za analizo smo uporabili analitsko tehtnico, konfokalni mikroskop, SEM in FTIR spektroskopijo. Pri tehtanju LDPE filma nismo opazili izgube mase, najverjetneje zaradi pritrjenega micelija in gojišča na film. S konfokalnim mikroskopom smo merili parameter hrapavost Sq ki predstavlja koren povprečja kvadratov višin. Če lahko zaupamo rezultatom, kljub visokim standardnim odklonom, smo ugotovili, da gliva Pleurotus ostreatus (Sq je 0,854 µm in standardni odklon je 0,350) po 30 dnevih povzroči večjo hrapavost, kot pa gliva Ceriporiopsis subvermispora (Sq je 0,678 µm in standardni odklon je 0,467). Iz tega lahko sklepamo da gliva Pleurotus ostreatus hitreje razgraja plastiko, kot pa Ceriporiopsis subvermispora. Hipoteze nismo uspeli potrditi ali ovršti, saj so bile meritve premalo natančne, da bi bili prepričani v naše rezultate.

Keywords

biotehnologija;glive;bela trohnoba;razgradnja polimerov;LDPE;sintetični polimeri;

Data

Language: Slovenian
Year of publishing:
Typology: 2.11 - Undergraduate Thesis
Organization: UL BF - Biotechnical Faculty
Publisher: [M. Kink]
UDC: 602.3:582.28:504.5:678.742.2(043.2)
COBISS: 125350659 Link will open in a new window
Views: 27
Downloads: 10
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Other data

Secondary language: English
Secondary title: Degredation of low density polyethylene with white rot fungi
Secondary abstract: Plastic pollution is one of the major problems of our time. Plastic has been found almost everywhere in the world, from the Mariana Trench to Mount Everest. It has a negative impact on the ecosystem, as animals die because of plastic waste. It has also been proven that plastic is already present in human bodies. In the bachelor thesis, we tried to find a better process for the degradation of low-density polyethylene with white rot fungi. We used the fungi Pleurotus ostreatus and Ceriporiopsis subvermispora. We hypothesized that fungi break down plastic faster in the presence of lignin in the culture medium. Analytical balance, confocal microscope, SEM and FTIR spectroscopy were used for analysis. No weight loss was observed when weighing the LDPE film, most likely due to the attached mycelium and culture medium to the film. Using a confocal microscope, we measured the roughness parameter Sq, which represents the root square mean of the height. If we can trust the results, despite the high standard deviations, we found that the fungus Pleurotus ostreatus (Sq is 0.854 µm and the standard deviation is 0.350) after 30 days causes a greater roughness than the fungus Ceriporiopsis subvermispora (Sq is 0.678 µm and the standard deviation is 0.467). From this we can conclude that the fungus Pleurotus ostreatus breaks down plastic faster than Ceriporiopsis subvermispora. We were unable to confirm or reject the hypothesis, as the measurements were insufficiently precise to be confident in our results.
Secondary keywords: biotechnology;fungi;plastic degradation;low density polyethylene;white rot fungi;
Type (COBISS): Bachelor thesis/paper
Study programme: 0
Embargo end date (OpenAIRE): 1970-01-01
Thesis comment: Univ. v Ljubljani, Biotehniška fak., Študij biotehnologije
Pages: 1 spletni vir (1 datoteka PDF (VIII, 18 f.))
ID: 16429400