magistrsko delo
Ana Brdnik (Author), Barbara Jeršek (Reviewer), Primož Treven (Mentor)

Abstract

Namen magistrske naloge je bil v vzorcih surovega mleka iz slovenskih mlekomatov raziskati prisotnost različnih genov, ki kodirajo odpornost proti tetraciklinom, eritromicinu, kloramfenikolu in aminoglikozidnim antibiotikom. V nalogi smo analizirali 50 vzorcev mleka, pri katerih smo z metodo PCR ugotavljali prisotnost genov za odpornost proti tetraciklinom (tet(A), tet(B), tet(C), tet(M), tet(O), tet(W)), eritromicinu (erm(A), erm(B), erm(C)), aminoglikozidnim antibiotikom (aac(6')-aph(2''), ant(6)-la, aph(3')-llla) in kloramfenikolu (cat). Največkrat smo ugotovili prisotnost gena aac(6')-aph(2'') in sicer v 21 vzorcih surovega mleka, sledijo mu preostala aminoglikozidna gena, tetraciklinski in eritromicinski geni, gena za odpornost proti kloramfenikolu nismo zaznali. Ugotovili smo tudi, da se pri analizi z metodo PCR meje zaznavnosti za posamezen gen razlikujejo in so v območju od 2 x 103 do 1 x 108 KE/mL mleka. Surovo mleko iz slovenskih mlekomatov vsebuje različne gene za odpornost proti antibiotikom, vendar še zdaleč ne toliko, kot poročajo iz drugih držav Evrope in sveta.

Keywords

surovo mleko;bakterije;odpornost proti antibiotikom;geni za odpornost proti antibiotikom;antibiotiki;

Data

Language: Slovenian
Year of publishing:
Typology: 2.09 - Master's Thesis
Organization: UL BF - Biotechnical Faculty
Publisher: [A. Brdnik Benčan]
UDC: 637.12:579.25:579.67
COBISS: 121939715 Link will open in a new window
Views: 21
Downloads: 13
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Other data

Secondary language: English
Secondary title: Presence of antibiotic resistance genes in raw milk samples from milk vending machines
Secondary abstract: The aim of the study was to investigate the presence of various genes encoding resistance against tetracyclines, erythromycin, chloramphenicol and aminoglycoside antibiotics in 50 samples of raw milk from Slovenian vending machines. By using PCR method we analysed the presence of genes encoding resistance to tetracyclines (tet(A), tet(B), tet(C), tet(M), tet(O), tet(W)), erythromycin (erm(A), erm(B), erm(C)), aminoglycoside antibiotics (aac(6')-aph(2''), ant(6)-la, aph(3')-llla) and chloramphenicol (cat). The most commonly determined antibiotic resistance gene was aac(6')-aph(2''), presented in 21 samples of raw milk, followed by the remaining aminoglycoside genes, tetracyclines and erythromycin genes. However, the gene encoding resistance to chloramphenicol was not detected. In addition, we found that in PCR method, selected antibiotic resistance genes had different limits of detection and were in range between 2 x 103 to 1 x 108 CFU/mL of milk. In conclusion, raw milk from Slovenian milk vending machines contains various antibiotic resistance genes, although the prevalence is lower compared to reports from other countries in Europe and world.
Secondary keywords: raw milk;bacteria;antibiotic resistance;antibiotic resistance genes;antibiotics;
Type (COBISS): Master's thesis/paper
Study programme: 0
Embargo end date (OpenAIRE): 1970-01-01
Thesis comment: Univ. v Ljubljani, Biotehniška fak., Oddelek za živilstvo
Pages: 1 spletni vir (1 datoteka PDF (IX, 46 str.))
ID: 16479188