ǂa ǂstudy on ET B knockout rats
Saranda Disha Ibrahimi (Author), Borut Furlani (Author), Gorazd Drevenšek (Author), Alja Meh (Author), M Yanagisawa (Author), Samo Hudoklin (Author), Irena Prodan Žitnik (Author), Janja Marc (Author), Martina Drevenšek (Author)

Abstract

The endothelin system has an important role in bone modelling during orthodontic tooth movement (OTM); however, little is known about the involvement of endothelin B receptors (ETB) in this process. The aim of this study was to evaluate the role of ETB in bone modelling during OTM using ETB knockout rats (ETB-KO). Thirty-two male rats were divided into 4 groups (n = 8 per group): the ETB-KO appliance group, ETB-KO control group, wild type (ETB-WT) appliance group, and ETB-WT control group. The appliance consisted of a super-elastic closed-coil spring placed between the first and second left maxillary molar and the incisors. Tooth movement was measured on days 0 and 35, and maxillary alveolar bone volume, osteoblast, and osteoclast volume were determined histomorphometrically on day 35 of OTM. Next, we determined the serum endothelin 1 (ET-1) level and gene expression levels of the osteoclast activity marker cathepsin K and osteoblast activity markers osteocalcin and dentin matrix acidic phosphoprotein 1 (DMP1) on day 35. The ETB-KO appliance group showed significantly lower osteoblast activity, diminished alveolar bone volume and less OTM than the ETB-WT appliance group. Our results showed that ETB is involved in bone modelling in the late stage of OTM.

Keywords

ortodontsko gibanje zob;endotelinski sistem;modeliranje kosti;orthodontic tooth movement;endothelin system;bone modelling;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UL MF - Faculty of Medicine
UDC: 616.31
COBISS: 27363587 Link will open in a new window
ISSN: 2045-2322
Views: 5
Downloads: 0
Average score: 0 (0 votes)
Metadata: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Other data

Secondary language: Slovenian
Secondary keywords: ortodontsko gibanje zob;endotelinski sistem;modeliranje kosti;
Type (COBISS): Article
Pages: str. [1-11]
Issue: ǂVol. ǂ10
Chronology: 2020
DOI: 10.1038/s41598-020-71159-8
ID: 16596529