diplomsko delo
Žan Marinković (Author), Matevž Pompe (Mentor)

Abstract

Pri diplomski nalogi sem razvijal metodo za določevanje vitamina D3 v prehranskih dopolnilih s pomočjo tekočinske kromatografije visoke ločljivosti. Metodo sem najprej optimiziral, tako da sem določil analizne pogoje (temperaturo, vrsto mobilnih faz, valovno dolžino, kolono, pretok, volumen injiciranja…) in metodi določil ustrezen gradient. Sledila je priprava različnih raztopin vzorcev in standardov z različnimi koncentracijami, snemanje in analiza njihovih kromatogramov. Pripravljene raztopine pa sem uporabil za validacijo svoje metode. Pri validaciji metode sem preveril njeno ponovljivost, linearnost ter stabilnost raztopin. Določil sem tudi LOQ in LOD (meja kvantifikacije in meja zaznave) za vitamin D3 in preveril kvantifikacijo s metodo standardnega dodatka. Izkazalo se je, da je ponovljivost metode dobra, stabilnost pripravljenih raztopin pa relativno slaba. Kvantifikacija z metodo standardnega dodatka je dajala podobne rezultate kot ob uporabi eksternega standarda, zato lahko predpostavimo točnost metode. Dobljena LOQ in LOD kažeta, da lahko vitamin D3 zaznamo in kvantificiramo pri zelo nizkih koncentracijah. Ugotovil sem, da je metoda za določanje vitamina D3 s pomočjo tekočinske kromatografije visoke ločljivosti primerna, vendar je potrebno takojšnje injiciranje po pripravi vzorca, ki vsebuje vitamin D3, saj če ga ne injiciramo takoj, začne vitamin D3 postopoma razpadati.

Keywords

prehranska dopolnila;vitamin D3;kromatografske metode;HPLC;optimizacija metode;validacija;diplomske naloge;

Data

Language: Slovenian
Year of publishing:
Typology: 2.11 - Undergraduate Thesis
Organization: UL FKKT - Faculty of Chemistry and Chemical Technology
Publisher: [Ž. Marinković]
UDC: 543.544.5HPLC(043.2)
COBISS: 134070787 Link will open in a new window
Views: 79
Downloads: 28
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Other data

Secondary language: English
Secondary title: Development of a method for determining vitamin D3 in food supplements
Secondary abstract: In my thesis, I have developed a method for determining vitamin D3 in dietary supplements using high-performing liquid chromatography. I first optimized the method by determining the analytical conditions (temperature, type of mobile phases, wavelength, column, flow, volume of injection…) and determined the appropriate gradient method. This was followed by the preparation of different solutions of samples and standards with different concentrations, filming and analysing their chromatograms. I used the prepared solutions to validate the method. During the validation of the method, I checked its repeatability, linearity and the stability of the solutions. I also determined the LOQ and LOD (limit of quantification and limit of detection) for vitamin D3 and checked the quantification using the standard additive method. It turns out that the repeatability of the method is good, but that the stability of the prepared solutions is relatively is poor. The quantification using the standard additive method gave similar results to that of the external standard, that is why we can assume the accuracy of the method. The resulting LOQ and LOD indicate that vitamin D3 can be detected and quantified at very low concentrations. I found out that the method for determining vitamin D3 using high-performance liquid chromatography is appropriate, but immediate injection is necessary after preparation of a sample containing vitamin D3, because if its not injected soon vitamin D3 starts to gradually decay.
Secondary keywords: vitamin D3;chromatography;HPLC;Tekočinska kromatografija;Univerzitetna in visokošolska dela;
Type (COBISS): Bachelor thesis/paper
Study programme: 1000374
Embargo end date (OpenAIRE): 1970-01-01
Thesis comment: Univ. v Ljubljani, Fak. za kemijo in kemijsko tehnologijo, VSŠ Kemijska tehnologija
Pages: 30 str.
ID: 16789221