magistrsko delo
Neja Frkal (Author), Tomaž Polak (Reviewer), Lea Pogačnik (Mentor), Tina Kosjek (Co-mentor)

Abstract

V magistrskem delu smo se osredotočili na identifikacijo fenolnih spojin v olupku korenine in mladih poganjkih japonskega dresnika (Fallopia japonica), sahalinskega dresnika (Fallopia sachalinensis) in češkega dresnika (Fallopia × bohemica). Nabrali smo rastlinski material in pripravili etanolne ekstrakte. S testom DPPH• smo določili njihovo antioksidativno učinkovitost, pri čemer so imeli ekstrakti olupkov korenin pri vseh treh taksonih troloksu ekvivalentno antioksidativno učinkovitost (TEAC) višjo od vrednosti v ekstraktih mladih poganjkov. Fenolne spojine v dresnikih smo identificirali z metodo tekočinske kromatografije ultra visoke ločljivosti, sklopljene z masnim spektrometrom tipa kvadrupol z detektorjem na čas preleta ionov. Ločbo fenolnih spojin smo dosegli z reverzno fazno kromatografijo, karakterizirali pa smo jih po ionizaciji z razprševanjem v električnem polju z visokoločljivostno in tandemsko masno spektrometrijo. Kot pomoč pri identifikaciji fenolnih spojin smo uporabili podatke iz masnospektrometričnih knjižnic, s čemer smo predlagali emodin-8-o-β-D-glukozid, procianidin B1, procianidin C1, taksifolin in epikatehin galat, za potrditev identitete pa smo uporabili referenčne standarde polifenolov in identificirali katehin oziroma epikatehin, polidatin, resveratrol, emodin, piceatanol, epigalokatehin galat, kvercetin, klorogensko kislino in ferulno kislino. Vsebnosti so se med ekstrakti razlikovale. Ugotovili smo, da je tekočinska kromatografija sklopljena z masno spektrometrijo ustrezna metoda za uspešno karakterizacijo fenolnih spojin in da so potrebne nadaljnje raziskave v smeri identifikacije in ocene vsebnosti polifenolov v dresnikih.

Keywords

dresnik;Fallopia;etanolni ekstrakt;antioksidativna učinkovitost;fenolne spojine;identifikacija;tekočinska kromatografija sklopljena z masno spektrometrijo;karakterizacija;resveratrol;katehin;emodin;

Data

Language: Slovenian
Year of publishing:
Typology: 2.09 - Master's Thesis
Organization: UL BF - Biotechnical Faculty
Publisher: [N. Frkal]
UDC: 547.56:582.665:543.51
COBISS: 143670019 Link will open in a new window
Views: 13
Downloads: 5
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Other data

Secondary language: English
Secondary title: Mass spectrometric characterization of extracts from invasive knotweed species
Secondary abstract: The aim of our study was to identify phenolic compounds in the root peels and young shoots of Japanese knotweed (Fallopia japonica), Giant knotweed (Fallopia sachalinensis) and Bohemian knotweed (Fallopia × bohemica). We collected plant material and prepared ethanol extracts of the samples. With DPPH• method we determined antioxidant efficiency of knotweed extract, where higher Trolox equivalent antioxidant capacity was determined in the root peel extracts compared to the young shoots ones. Separation of phenolic compounds was performed by reverse phase chromatography followed by ionization with scattering in an electric field. We then characterized the phenolic compounds with high resolution and tandem mass spectrometry. We used data from mass spectrometric libraries as a tool for identification where we proposed emodin-8-o-β-D-glucoside, procyanidin B1, procyanidin C1, taxifolin and epicatechin gallate. We confirmed the compounds with reference standards of phenolic compounds. We identified catechin or epicatechin, polydatin, resveratrol, emodin, piceatanol, epigallocatechin gallate, quercetin, chlorogenic acid and ferulic acid. Content of compounds among extracts is different. We found that liquid chromatography coupled with mass spectrometry is a useful method for the successful identification and characterization of phenolic compounds and more research for identification and characterization in knotweed is needed.
Secondary keywords: knotweed;Fallopia;ethanol extracts;antioxidant efficiency;phenolic compounds;identification;characterization;liquid chromatography coupled with mass spectrometry;resveratrol;catechin;emodin;
Type (COBISS): Master's thesis/paper
Study programme: 0
Thesis comment: Univ. v Ljubljani, Biotehniška fak., Oddelek za živilstvo
Pages: 1 spletni vir (1 datoteka PDF (XI, 59 str., [17] f. pril.))
ID: 18131194