Katarina Šimunović (Author), Orhan Sahin (Author), Andi Erega (Author), Polonca Štefanič (Author), Qijing Zhang (Author), Ines Mandić-Mulec (Author), Sonja Smole Možina (Author), Anja Klančnik (Author)

Abstract

Campylobacter jejuni is the leading cause of bacterial gastroenteritis, or campylobacteriosis, in humans worldwide, and poultry serves as a major source of infection. To reduce the risk associated with C. jejuni transmission via poultry meat, effective interventions during poultry production are needed, and the use of probiotics is a promising approach. In this study, 15 Bacillus subtilis strains were initially screened for their anti-Campylobacter activities. B. subtilis PS-216 strain demonstrated the best anti-Campylobacter activity against 15 C. jejuni isolates when examined using in vitro co-cultures. To evaluate the suitability of B. subtilis PS-216 for probiotic use, its susceptibility to eight clinically important antimicrobials and simulated gastric conditions was investigated. B. subtilis PS-216 was sensitive to all of the tested antibiotics. Although vegetative cells were sensitive to gastric conditions, B. subtilis PS-216 spores were highly resistant. We further evaluated the use of a B. subtilis PS-216 spore preparation (2.5 × 106 CFU/mL water) to prevent and/or reduce C. jejuni colonization in broiler chickens in vivo. Compared to the untreated group, significantly lower Campylobacter counts were detected in caeca of broilers continuously treated with B. subtilis PS-216 spores in their drinking water. Furthermore, broilers continuously treated with B. subtilis PS-216 spores showed improved weight gain, compared to the control group. Together, these results demonstrate the potential of B. subtilis PS-216 for use in poultry to reduce C. jejuni colonization and improve weight gain.

Keywords

probiotiki;inhibicija rasti;sporogene bakterije;perutnina;Campylobacter jejuni;Bacillus subtilis;probiotics;alternative to antibiotics;spore-containing drinking water;broiler chicken;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UL BF - Biotechnical Faculty
UDC: 579.22/.26:579.67
COBISS: 115015683 Link will open in a new window
ISSN: 1664-302X
Views: 101
Downloads: 32
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Other data

Secondary language: Slovenian
Secondary keywords: probiotiki;inhibicija rasti;sporogene bakterije;perutnina;
Type (COBISS): Article
Pages: str. 1-11
Issue: ǂVol. ǂ13
Chronology: Jul. 2022
DOI: 10.3389/fmicb.2022.910616
ID: 18625363